■核因子类红细胞2相关因子2(Nrf2)/血红素加氧酶-1(HO-1)信号通路在调节抗氧化和抗病毒反应中的作用已被广泛研究。马科疱疹病毒8型(EqHV-8)对马业构成重大威胁,主要表现为呼吸道疾病,堕胎,马和驴的神经系统疾病。氧化应激被认为是与EqHV-8感染发病相关的关键因素。不幸的是,目前缺乏有效控制EqHV-8的治疗性干预措施.芦丁的抗氧化和抗病毒潜力已得到充分证明。在当前的研究中,我们专注于评估芦丁作为抗EqHV-8感染的潜在治疗剂。
■为此,我们包括体外和体内研究,以评估芦丁对抗EqHV-8感染的有效性.
■从体外实验获得的结果表明,芦丁在病毒生命周期的多个阶段对EqHV-8具有明显的抑制作用。通过细致的实验,我们阐明了芦丁对EqHV-8的抗病毒作用与Nrf2/HO-1信号通路介导的抗氧化反应密切相关。发现芦丁对该途径的激活显着阻碍了EqHV-8的复制,从而减少病毒载量。这种机制见解不仅增强了我们对芦丁抗病毒潜力的理解,而且还突出了抗氧化应激反应在对抗EqHV-8感染中的重要性。为了补充我们的体外研究结果,我们使用小鼠模型进行了体内研究。这些实验表明,芦丁给药导致小鼠肺部EqHV-8感染的大幅减少,强调该化合物在体内的治疗前景。
■求和,我们的发现表明,芦丁有望成为预防和控制EqHV-8感染的新型有效治疗剂。
UNASSIGNED: The Nuclear factor erythroid 2-related factor 2 (Nrf2)/heme oxygenase-1 (HO-1) signaling pathway has been extensively studied for its role in regulating antioxidant and antiviral responses. The Equid herpesvirus type 8 (EqHV-8) poses a significant threat to the equine industry, primarily manifesting as respiratory disease, abortions, and neurological disorders in horses and donkeys. Oxidative stress is considered a key factor associated with pathogenesis of EqHV-8 infection. Unfortunately, there is currently a dearth of therapeutic interventions available for the effective control of EqHV-8. Rutin has been well documented for its antioxidant and antiviral potential. In current study we focused on the evaluation of Rutin as a potential therapeutic agent against EqHV-8 infection.
UNASSIGNED: For this purpose, we encompassed both in-vitro and in-vivo investigations to assess the effectiveness of Rutin in combatting EqHV-8 infection.
UNASSIGNED: The results obtained from in vitro experiments demonstrated that Rutin exerted a pronounced inhibitory effect on EqHV-8 at multiple stages of the viral life cycle. Through meticulous experimentation, we elucidated that Rutin\'s antiviral action against EqHV-8 is intricately linked to the Nrf2/HO-1 signaling pathway-mediated antioxidant response. Activation of this pathway by Rutin was found to significantly impede EqHV-8 replication, thereby diminishing the viral load. This mechanistic insight not only enhances our understanding of the antiviral potential of Rutin but also highlights the significance of antioxidant stress responses in combating EqHV-8 infection. To complement our in vitro findings, we conducted in vivo studies employing a mouse model. These experiments revealed that Rutin administration resulted in a substantial reduction in EqHV-8 infection within the lungs of the mice, underscoring the compound\'s therapeutic promise in vivo.
UNASSIGNED: In summation, our finding showed that Rutin holds promise as a novel and effective therapeutic agent for the prevention and control of EqHV-8 infections.