锑(Sb)是一种在自然环境中广泛分布的有毒准金属。微生物,尤其是真菌,可以作为生物修复锑污染土壤和水域的理想生物材料。在这项研究中,我们分离出了一种抗锑真菌,从中国西匡山锑矿收集的矿渣样品中提取的石榴石ZJ-1。ZJ-1对Sb表现出极高的抗性,亚砷酸盐[Sb(Ⅲ)]的MIC水平>175mM,砷酸盐[Sb(V)]的MIC水平为40mM。全基因组分析确定了ZJ-1基因组上的多个Sb(Ⅲ)和/或As(Ⅲ)抗性基因,这可以部分解释其对Sb的高抗性。还定量了ZJ-1从Sb(Ⅲ)或Sb(V)溶液中去除Sb的潜力。ZJ-1对Sb(Ⅲ)和Sb(V)的平均吸附容量分别为635.14mg/g和149.65mg/g,分别,在Sb水溶液中,初始浓度为2000mg/L(16.43mM)。此外,生长系统中的Sb(Ⅲ)在初始浓度为500mg/L(4.11mM)时被去除近99%。此外,利用傅里叶变换红外光谱(FT-IR)和X射线光电子能谱(XPS)研究了Sb在ZJ-1和-OH,-NH2,-COOH,发现C-O和C-O-C是ZJ-1细胞吸附Sb的主要表面官能团。
Antimony (Sb) is a toxic metalloid widely distributed in the natural environments. Microorganisms, especially fungi, could serve as ideal biomaterials for bioremediation of Sb-polluted soils and waters. In this study, we isolated an antimony-resistant fungus, Sarocladium kiliense ZJ-1, from a slag sample collected in Xikuangshan Sb mine in P. R. China. ZJ-1 showed an extremely high resistance to Sb, with a MIC level of > 175 mM for arsenite [Sb(Ⅲ)] and 40 mM for arsenate [Sb(V)]. Whole genomic analysis identified multiple Sb (Ⅲ)- and/or As(Ⅲ)-resistant genes on ZJ-1\'s genome, which may partially explain its hyper-resistance to Sb. The potential of ZJ-1 in removing Sb from Sb(Ⅲ) or Sb(V) solutions was also quantified. The average biosorption capacity of ZJ-1 for Sb(Ⅲ) and Sb(V) is 635.14 mg/g and 149.65 mg/g, respectively, in Sb aqueous solutions with an initial concentration of 2000 mg/L (16.43 mM). Besides, almost 99% of Sb(Ⅲ) in the growing system was removed with an initial concentration of 500 mg/L (4.11 mM). Furthermore, Fourier transformation infrared spectroscopy (FT-IR) and X-ray photoelectron spectroscopy (XPS) were used to probe the Sb adsorption mechanism on ZJ-1, and -OH, -NH2, -COOH, C-O and C-O-C were found to be the main surface functional groups of ZJ-1 cells to adsorb Sb.