未经批准:五价抗生素一直是治疗利什曼病的首选药物,尽管出现了治疗失败。迫切需要鉴定抗性标记以设计新的治疗策略。铁-超氧化物歧化酶(Fe-SODs)是抗氧化酶,有助于使活性氧物质解毒以防止细胞受到氧化应激。由于锑化合物诱导氧化应激,在这次调查中,研究了SOD基因的表达,以确定其在临床耐药菌株中的表达模式。
未经调查:这项横断面调查是在马什哈德市进行的,2014年至2019年期间位于伊朗东北部。在25例锑反应性(n=15)和无反应性(n=10)的降指性皮肤利什曼病(ACL)患者中研究了线粒体(SODA)和糖体(SODB)超氧化物歧化酶的RNA表达水平。总RNA提取和cDNA合成,qRT-PCR方法用于研究相对RNA表达水平。
未经鉴定:在抗性最强的分离株中,SODs的转录水平过表达。基因表达分析表明,SODA和B的过表达分别为3.8和4.81,在抗性分离株与敏感的。
未经证实:无反应寄生虫中SODA/B的异常表达可能与锑诱导的氧化应激解毒有关。此外,SODs可能被认为是流行地区ACL患者对抗生素反应的潜在预测指标。
UNASSIGNED: Pentavalent antimonial has been a drug of choice against leishmaniasis, despite the emergence of treatment failure. Identification of resistance markers is urgently needed to design new therapeutic strategies. Iron-Superoxide dismutases (Fe-SODs) are antioxidant enzymes contributing to detoxify reactive oxygen species to prevent a cell from oxidative stress. Since antimonial compounds induce oxidative stress, in this survey, the expression of SOD genes was investigated to identify their expression pattern in clinical resistant isolates.
UNASSIGNED: This cross-sectional survey was done in Mashhad City, northeast of Iran during 2014 to 2019. The RNA expression level of mitochondrial (SODA) and glycosomal (SODB) superoxide dismutase was investigated in 25 antimony responsive (n=15) and unresponsive (n=10) anthroponotic cutaneous leishmaniasis (ACL) patients. Total RNA extraction and cDNA synthesis, the qRT-PCR approach was utilized to investigate the relative RNA expression level.
UNASSIGNED: The transcript level of SODs was over-expressed in the most resistant isolates. Gene expression analysis demonstrated the over-expression of SODA and B by a factor of 3.8 and 4.81, respectively, in resistance isolates vs. sensitive ones.
UNASSIGNED: Aberrant expression of SODA/B in unresponsive parasites could potentially implicate in detoxifying antimony-induced oxidative stress. Moreover, SODs might be considered as potential predictive markers of the response to antimonials in ACL patients in endemic areas.