■多柔比星(DOX)作为化疗药物的使用由于其心脏毒性作用而受到限制。鼠尾草酸发挥抗氧化作用,抗炎,除了细胞保护作用.本研究的目的是研究鼠尾草酸保护大鼠心脏和MCF7细胞系免受DOX诱导的心脏毒性的能力。
■该研究涉及将雄性Wistar大鼠分为七个组:1)对照2)DOX(2mg/kg,每48h,IP,12d),3-5)鼠尾草酸(10、20、40mg/kg/天,IP,16d)+DOX,6)维生素E(200mg/kg,每48h,IP,16d)+DOX7)鼠尾草酸(40毫克/千克/天,IP,16d).最后,心脏组织病理学改变,心电图因素,颈动脉血压,左心室功能,心脏体重比,氧化(MDA,GSH),炎性(IL-1β,TNF-α),再加上细胞凋亡(caspase3,8,9,Bcl-2,Bax)标记进行评估。采用MTT法评价DOX对MCF7细胞的毒性和鼠尾草酸的改善作用。
■DOX增加了QRS持续时间,QA,RRI,STI,和心脏体重比,减少HR,LVDP,最小dP/dt,最大dP/dt,血压,增强的MDA,TNF-α,IL1-β,caspase3,8,9,Bax/Bcl-2比值,GSH含量降低,引起的纤维化,坏死,和心脏组织中的细胞质空泡化,但鼠尾草酸的给药降低了DOX的毒性作用。浓度为5和10μM的鼠尾草酸不影响DOX的细胞毒性作用。
■鼠尾草酸作为抗炎和抗氧化物质,通过增强抗氧化防御和改变炎症信号通路活性,可有效减少DOX诱导的损伤,可作为治疗DOX心脏毒性的辅助手段。
UNASSIGNED: Utilization of doxorubicin (DOX) as a chemotherapy medication is limited due to its cardiotoxic effects. Carnosic acid exerts antioxidant, anti-inflammatory, besides cytoprotective effects. The objective of this study was to investigate the ability of carnosic acid to protect rat hearts and the MCF7 cell line against cardiotoxicity induced by DOX.
UNASSIGNED: The study involved the classification of male Wistar rats into seven groups: 1) Control 2) DOX (2 mg/kg, every 48h, IP, 12d), 3-5) Carnosic acid (10, 20, 40 mg/kg/day, IP, 16d)+ DOX, 6) Vitamin E (200 mg/kg, every 48h, IP, 16d)+ DOX 7) Carnosic acid (40 mg/kg/day, IP, 16d). Finally, cardiac histopathological alterations, ECG factors, carotid blood pressure, left ventricular function, heart-to-body weight ratio, oxidative (MDA, GSH), inflammatory (IL-1β, TNF-α), plus apoptosis (caspase 3, 8, 9, Bcl-2, Bax) markers were evaluated. DOX toxicity and carnosic acid ameliorative effect were evaluated on MCF7 cells using the MTT assay.
UNASSIGNED: DOX augmented the QRS duration, QA, RRI, STI, and heart-to-body weight ratio, and reduced HR, LVDP, Min dP/dt, Max dP/dt, blood pressure, boosted MDA, TNF-α, IL1-β, caspase 3,8,9, Bax/Bcl-2 ratio, decreased GSH content, caused fibrosis, necrosis, and cytoplasmic vacuolization in cardiac tissue but carnosic acid administration reduced the toxic effects of DOX. The cytotoxic effects of DOX were not affected by carnosic acid at concentrations of 5 and 10 μM.
UNASSIGNED: Carnosic acid as an anti-inflammatory and antioxidant substance is effective in reducing DOX-induced damage by enhancing antioxidant defense and modifying inflammatory signal pathway activity and can be used as an adjunct in treating DOX cardiotoxicity.