通过DEAE-52纤维素和SephacrylS-500柱色谱从天祝仙岳炒绿茶中分离纯化了一种新的均质多糖(TPS3A)。结构表征表明TPS3A主要由阿拉伯糖组成,半乳糖,半乳糖醛酸和鼠李糖的摩尔比为5.84:4.15:2.06:1,平均分子量为1.596×104kDa。TPS3A的结构表征为由1,3-Galp组成的重复单元,1,4-Galp,1,3,6-Galp,1,3-Araf,1,5-Araf,1,2,4-Rhap和1-GalpA,在1,3,6-Galp的C6和1,2,4-Rhap的C2上有两个分支,分别。探讨TPS3A对动脉粥样硬化的预防作用,将TPS3A口服施用于ApoE缺陷型(ApoE-/-)小鼠。结果提示TPS3A干预能有效延缓动脉粥样硬化斑块进展,调节血脂异常,并通过激活高脂饮食诱导的ApoE-/-小鼠中收缩标记α-平滑肌肌动蛋白(α-SMA)的表达和抑制合成标记骨桥蛋白(OPN)的表达,减少血管平滑肌细胞(VSMCs)从收缩表型向合成表型的转化。我们的发现表明,TPS3A通过调节VSMC的血脂异常和表型转变显着减轻动脉粥样硬化,并可能用作促进心血管健康的新型功能成分。
A new homogeneous polysaccharide (TPS3A) was isolated and purified from Tianzhu Xianyue fried green tea by DEAE-52 cellulose and Sephacryl S-500 column chromatography. Structural characterization indicated that TPS3A mainly consisted of arabinose, galactose, galacturonic acid and rhamnose in a molar ratio of 5.84: 4.15: 2.06: 1, with an average molecular weight of 1.596 × 104 kDa. The structure of TPS3A was characterized as a repeating unit consisting of 1,3-Galp, 1,4-Galp, 1,3,6-Galp, 1,3-Araf, 1,5-Araf, 1,2,4-Rhap and 1-GalpA, with two branches on the C6 of 1,3,6-Galp and C2 of 1,2,4-Rhap, respectively. To investigate the preventive effects of TPS3A on atherosclerosis, TPS3A was administered orally to ApoE-deficient (ApoE-/-) mice. Results revealed that TPS3A intervention could effectively delay the atherosclerotic plaque progression, modulate dyslipidemia, and reduce the transformation of vascular smooth muscle cells (VSMCs) from contractile phenotype to synthetic phenotype by activating the expression of contractile marker alpha-smooth muscle actin (α-SMA) and inhibiting the expression of synthetic marker osteopontin (OPN) in high-fat diet-induced ApoE-/- mice. Our findings suggested that TPS3A markedly alleviated atherosclerosis by regulating dyslipidemia and phenotypic transition of VSMCs, and might be used as a novel functional ingredient to promote cardiovascular health.