■几十年来,射频(RF)诱导的组织融合由于其替代缝合线和U形钉用于组织重建吻合的潜力而引起了极大的关注。然而,实现高结合强度和减少过度热损伤的复杂性对现有的熔合装置提出了实质性的限制。
■本研究提出了一种离散的连锁型电极,用于进行离体RF诱导的肠吻合实验。通过爆裂压力和剪切强度测试检查吻合强度。通过红外热成像仪监测热损伤的程度。通过组织病理学和超微结构观察,进一步研究了电极融合的吻合口。
■吻合组织的爆裂压力和剪切强度可达到62.2±3.08mmHg和8.73±1.11N,分别,当压力,功率和持续时间为995kPa,160瓦和13秒,和热损伤可以控制在限度内。组织病理学和超微结构观察表明,可以形成完整且完全融合的气孔,并具有胶原交联。
■离散连接型电极在射频诱导的组织融合中具有良好的效率和安全性,这些结果为具有可控压力和能量输送的电外科医疗设备的设计提供了信息。
UNASSIGNED: For decades, radiofrequency (RF)-induced tissue fusion has garnered great attention due to its potential to replace sutures and staples for anastomosis of tissue reconstruction. However, the complexities of achieving high bonding strength and reducing excessive thermal damage present substantial limitations of existing fusion devices.
UNASSIGNED: This study proposed a discrete linkage-type electrode to carry out ex vivo RF-induced intestinal anastomosis experiments. The anastomotic strength was examined by burst pressure and shear strength test. The degree of thermal damage was monitored through an infrared thermal imager. And the anastomotic stoma fused by the electrode was further investigated through histopathological and ultrastructural observation.
UNASSIGNED: The burst pressure and shear strength of anastomotic tissue can reach 62.2 ± 3.08 mmHg and 8.73 ± 1.11N, respectively, when the pressure, power and duration are 995 kPa, 160 W and 13 s, and the thermal damage can be controlled within limits. Histopathological and ultrastructural observation indicate that an intact and fully fused stomas with collagenic crosslink can be formed.
UNASSIGNED: The discrete linkage-type electrode presents favorable efficiency and security in RF-induced tissue fusion, and these results are informative to the design of electrosurgical medical devices with controllable pressure and energy delivery.