在厌氧氧化过程中，缓解生物质冲洗以增加污泥保留是过程效率的重要参数。信号分子刺激剂(SMS)引发由微生物的程序性细胞死亡(PCD)控制的污泥粒化。在这项研究中,好氧颗粒污泥(AGS)，细胞碎片,胞外聚合物（EPS），和AGS工艺废水作为SMS进行测试，以确定它们对厌氧氨氧化造粒的影响。结果表明,SMS的加入不同程度地提高了脱氮效率,而添加AGS工艺上清液，作为SMS，氨去除效率提高到96%。还发现添加SMS可增加EPS产量并有助于污泥颗粒化。在这个过程中,PCD的比例增加，Gaiella和Denitratisoma的丰度从3.54％增加到5.59％，从1.8%到3.42%，分别。总之,发现PCD通过造粒机理对提高厌氧氨氧化性能很重要。
During the anammox process, mitigation of biomass washout to increase sludge retention is an important parameter of process efficiency. Signal molecular stimulants (SMS) initiate the sludge granulations controlled by programmed cell death (PCD) of microorganisms. In this study, the aerobic granular sludge (AGS), cell fragments, extracellular polymeric substances (EPS), and AGS process effluent were tested as SMS to identify their effect on anammox granulation. The results showed that the addition of SMS increased the nitrogen removal efficiency to varying degrees, whereas the addition of AGS process supernatant, as SMS, increased the ammonia removal efficiency up to 96%. The addition of SMS was also found to increase EPS production and contributed to sludge granulation. In this process, the proportion of PCD increased and both Gaiella and Denitratisoma abundance increased from 3.54% to 5.59%, and from 1.8% to 3.42%, respectively. In conclusion, PCD was found important to increase anaerobic ammonia oxidation performance through the granulation mechanism.
