锆金属有机骨架UiO-66-NH2因其从水中去除环境污染物的潜力而引起了广泛关注。UiO-66-NH2的生产和应用使其不可避免地释放到水生环境中。然而,关于其对环境和水生生物的潜在风险的信息很少,从而限制了对其安全和可持续使用的评估。在这项研究中,评估了UiO-66-NH2的生态毒性,特别是它对增长的影响,细胞外有机质释放,和模型浮游植物铜绿微囊藻的代谢组学变化(M.铜绿假单胞菌)。UiO-66-NH2对藻类生理包括生长表现出中等影响,生存能力,和光合系统。在浓度低于20mg/L时,UiO-66-NH2对藻类生长的抑制作用可忽略不计,藻类生存能力,和光合作用。相比之下,UiO-66-NH2即使在低至0.02mg/L的浓度下也能促进细胞外有机物的释放。这些研究结果表明,虽然没有观察到对藻类细胞的明显损害,UiO-66-NH2对水生环境有害,因为它刺激了藻类毒素的释放。此外,如通过荧光成像观察到的,UiO-66-NH2进入藻类细胞而不是粘附到铜绿分枝杆菌的表面。基于代谢分析,UiO-66-NH2主要通过干扰嘌呤代谢和ABC转运体来影响蓝藻。本研究揭示了UiO-66-NH2对微藻的潜在威胁,并对其在环境领域的安全利用具有潜在的影响。
The zirconium metal-organic framework UiO-66-NH2 has garnered considerable attention for their potentials of removing environmental contaminants from water. The production and application of UiO-66-NH2 make their releases into the aquatic environment inevitable. Nevertheless, little information is available regarding its potential risk to the environment and aquatic organisms, thus limiting the evaluation of its safe and sustainable use. In this study, the ecotoxicity of UiO-66-NH2 was evaluated, specifically its impacts on growth, extracellular organic matter release, and metabolomic changes of the model phytoplankton Microcystis aeruginosa (M. aeruginosa). UiO-66-NH2 exhibited moderate effects on algal physiology including growth, viability, and photosynthetic system. At concentrations below 20 mg/L, UiO-66-NH2 induced negligible inhibition of algal growth, algal viability, and photosynthesis. In contrast, UiO-66-NH2 boosted the release of extracellular organic matter even at concentration as low as 0.02 mg/L. These findings indicated that, while no evident damage to algal cells was observed, UiO-66-NH2 was hazardous to the aquatic environment as it stimulated the release of algal toxins. Moreover, UiO-66-NH2 entered algal cells rather than adhering to the surface of M. aeruginosa as observed by the fluorescence imaging. Based on metabolic analysis, UiO-66-NH2 influenced the cyanobacteria mainly through interference with purine metabolism and ABC transporter. This study sheds light on the potential threat UiO-66-NH2 posing to microalgae, and has potential implications for its safe utilization in the environmental field.