背景:随着全球人口老龄化,老年人败血症的患病率增加,特别容易发炎的人口统计学。这项研究旨在评估氢气的治疗潜力,以其抗炎和抗氧化特性而闻名,在减轻炎症,特别是在肺和肝脏,和老年小鼠的年龄相关分子标记。
方法:21至23个月的雄性小鼠,代表人类老年人口,通过腹膜内注射脂多糖(LPS)进行炎症。将小鼠分为八组,以检查不同持续时间和浓度的氢气吸入的影响:对照组,不含氢的盐水,含24小时2%氢气的盐水,不含氢的LPS,含24小时2%氢气的LPS,LPS与6小时2%氢气,含1小时2%氢气的LPS,和具有24小时1%氢气的LPS。评估的参数包括生存率,活动水平,炎症生物标志物,器官损伤。
结果:通过减少肺和肝组织中炎性生物标志物的mRNA表达,延长氢气浓度为2%24小时的老年小鼠预后良好。减轻肺损伤,并减少衰老相关蛋白p21的表达。此外,氢气吸入选择性改善肺组织衰老相关标志物,包括C-X-C基序趋化因子2、金属蛋白酶-3和精氨酸酶-1。值得注意的是,在测试条件下,氢气不能减轻LPS诱导的肝损伤。
结论:该研究强调,以2%浓度连续吸入氢气24小时可以通过减轻肺部炎症和调节衰老来改善老年人群的生存和体力活动。与LPS诱导的炎症相关的标志物。这一发现为未来研究氢气作为缓解可导致老年人器官损伤的严重炎症的治疗策略铺平了道路。
With the global population aging, there is an increased prevalence of sepsis among the elderly, a demographic particularly susceptible to inflammation. This study aimed to evaluate the therapeutic potential of hydrogen gas, known for its anti-inflammatory and antioxidant properties, in attenuating inflammation specifically in the lungs and liver, and age-associated molecular markers in aged mice.
Male mice aged 21 to 23 months, representative of the human elderly population, were subjected to inflammation via intraperitoneal injection of lipopolysaccharide (LPS). The mice were allocated into eight groups to examine the effects of varying durations and concentrations of hydrogen gas inhalation: control, saline without hydrogen, saline with 24-hour 2 % hydrogen, LPS without hydrogen, LPS with 24-hour 2 % hydrogen, LPS with 6-hour 2 % hydrogen, LPS with 1-hour 2 % hydrogen, and LPS with 24-hour 1 % hydrogen. Parameters assessed included survival rate, activity level, inflammatory biomarkers, and organ injury.
Extended administration of hydrogen gas specifically at a 2 % concentration for 24 h led to a favorable prognosis in the aged mice by reducing mRNA expression of inflammatory biomarkers in lung and liver tissue, mitigating lung injury, and diminishing the expression of the senescence-associated protein p21. Moreover, hydrogen gas inhalation selectively ameliorated senescence-related markers in lung tissue, including C-X-C motif chemokine 2, metalloproteinase-3, and arginase-1. Notably, hydrogen gas did not alleviate LPS-induced liver injury under the conditions tested.
The study highlights that continuous inhalation of hydrogen gas at a 2 % concentration for 24 h can be a potent intervention in the geriatric population for improving survival and physical activity by mitigating pulmonary inflammation and modulating senescence-related markers in aged mice with LPS-induced inflammation. This finding paves the way for future research into hydrogen gas as a therapeutic strategy to alleviate severe inflammation that can lead to organ damage in the elderly.