我们介绍了5,10,15,20-四苯基卟啉二氯化锡(IV)(Sn-tpp)作为杂合蛋白标记的潜在用途的电化学和光学表征。我们的研究包括Sn-tpp和Sn-tpp在模型蛋白的存在下选择作为模拟受体或表面阻断剂:牛血清白蛋白,卵白蛋白,和免疫球蛋白G。在研究过程中,我们通过差分脉冲伏安法确定了分析的最佳条件,紫外-可见分光光度法,和荧光光谱法。在电化学检测中,工作电极的影响,溶剂,并检查了支持电解质。观察并分析了接收信号沿电位轴的位移(电位的偏移)和由于蛋白质的添加而引起的信号强度的变化。同时,通过使用光谱技术评估Sn-tpp作为光学检测模式下的标记的适用性。获得的结果证明Sn-tpp适用于双重和三重检测系统。这种方法将提高分析的可靠性,同时,将允许扩大线性响应的范围与一些重叠的浓度范围。
We present an electrochemical and optical characterization of 5,10,15,20-tetraphenylporphyrin tin(IV) dichloride (Sn-tpp) in terms of its potential use as a hybrid proteins\' label. Our research comprised Sn-tpp and Sn-tpp in the presence of model proteins selected as to mimic a receptor or surface blocking agents: bovine serum albumin, ovalbumin, and immunoglobulin G. In the course of the study, we determined optimal conditions for analysis by means of differential pulse voltammetry, ultraviolet-visible spectrophotometry, and spectrofluorimetry. In electrochemical detection, the influence of the working electrode, solvent, and supporting electrolyte was examined. Displacements of the received signals along the potential axis (a shift of the potential) and changes in signal intensities due to the addition of proteins were observed and analyzed. Simultaneously, the suitability of Sn-tpp as a label in optical detection mode was assessed by using spectroscopic techniques. The obtained results prove Sn-tpp to be applicable in dual and triple detection systems. Such an approach will improve the reliability of the analysis and, at the same time, will allow for widening the range of the linear response with some overlapping ranges of concentrations.