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Abstract：
Biosensors play a central role in moving diagnostics to being on-site or decentralized. Affinity biosensor, an important category of biosensors, has important applications in clinical diagnosis, pharmaceuticals, immunology, and other fields. Affinity biosensors rely on specific binding between target analytes and biological ligands such as antibodies, nucleic acids, or other receptors to generate measurable signals. Oftentimes the target analytes in practical samples are of low abundance in a complex matrix. Traditional affinity biosensors mainly rely on random diffusion of analytes in solution to conjugate with biorecognition elements on the sensor surface of electrodes. The process may take hours or even days, which is not conducive to rapid and sensitive detection of biosensors. Therefore, it is strongly desired to incorporate an enrichment mechanism for target analytes into biosensor-based detection. AC electrokinetic (ACEK) effect can realize rapid enrichment of analytes by application of AC electric fields, which holds great promise for achieving high sensitivity, low detection limit, and rapid turnaround. This article reviews the studies of affinity biosensors integrated with ACEK enrichment in the past decade, and summarizes the latest detection methods, detection devices and applications, hoping to provide some insights and references for researchers in related fields.
摘要：
生物传感器在将诊断转移到现场或分散方面发挥着核心作用。亲和生物传感器，生物传感器的一个重要类别，在临床诊断中有着重要的应用,制药，免疫学,和其他领域。亲和生物传感器依赖于目标分析物和生物配体如抗体之间的特异性结合，核酸,或其他受体来产生可测量的信号。通常,实际样品中的目标分析物在复杂基质中具有低丰度。传统的亲和生物传感器主要依靠分析物在溶液中的随机扩散与电极的传感器表面上的生物识别元素缀合。这个过程可能需要几个小时甚至几天，不利于生物传感器的快速、灵敏检测。因此,强烈希望将目标分析物的富集机制并入基于生物传感器的检测中。交流电动(ACEK)效应可以通过施加交流电场实现分析物的快速富集,这对实现高灵敏度有着巨大的希望，低检测限，和快速周转。本文回顾了近10年来与ACEK富集集成的亲和生物传感器的研究。并总结了最新的检测方法，检测设备和应用，希望能为相关领域的研究者提供一些见解和参考。
