循环肿瘤细胞(CTCs)在肿瘤转移中发挥重要作用,这与死亡风险增加呈正相关。肌动蛋白结合蛋白,包括cofilin(CFL1),profilin1(PFN1),和腺苷酸环化酶相关蛋白1(CAP1),被认为与肿瘤细胞运动和转移有关,特别是在头颈部鳞状细胞癌(HNSCC)。然而,目前,目前尚无关于CFL1,PFN1和CAP1在CTC和HNSCC患者白细胞中的发表研究.我们评估了31例HNSCC患者(T1-4N0-2M0)血液中CFL1,PFN1和CAP1的血清水平以及包含这些蛋白质的CTC和白细胞的数量。分析使用流式细胞术和酶联免疫吸附测定试剂盒。我们发现CAP1+CTC和CAP1+白细胞亚群在这些HNSCC患者样本中普遍存在,而CFL1+和PFN1+CTC的患病率相对较低。T2-4N1-2M0期患者有CFL1+和PFN1+CTC,PFN1血清水平升高,与T1-3N0M0组比拟。总之,PFN1血清水平和PFN1+CD326+CTC的相对数量可能是HNSCC转移的有价值的预后标志物。当前的研究是第一个获得有关CTC中肌动蛋白结合蛋白(ABP)含量的数据,和HNSCC患者血液中的白细胞。这也是初次考核CTCs亚群数目与疾病特点之间的关系。
Circulating tumor cells (CTCs) play an important role in tumor metastases, which is positively correlated with an increased risk of death. Actin-binding proteins, including cofilin (CFL1), profilin 1 (PFN1), and adenylate cyclase-associated protein 1 (CAP1), are thought to be involved in tumor cell motility and metastasis, specifically in head and neck squamous cell carcinoma (HNSCC). However, currently, there are no published studies on CFL1, PFN1, and CAP1 in CTCs and leukocytes in HNSCC patients. We assessed serum levels of CFL1, PFN1, and CAP1 and the number of CTCs and leukocytes containing these proteins in blood from 31 HNSCC patients (T1-4N0-2M0). The analysis used flow cytometry and an enzyme-linked immunosorbent assay kit. We found that CAP1 + CTCs and CAP1 + leukocyte subpopulations were prevalent in these HNSCC patient samples, while the prevalence rates of CFL1 + and PFN1 + CTCs were relatively low. Patients with stage T2-4N1-2M0 had CFL1 + and PFN1 + CTCs with an elevated PFN1 serum level, compared with the T1-3N0M0 group. In summary, the PFN1 serum level and the relative number of PFN1 +CD326 + CTCs could be valuable prognostic markers for HNSCC metastases. The current study is the first to obtain data regarding the contents of actin-binding proteins (ABPs) in CTCs, and leukocytes in blood from HNSCC patients. This is also the first to assess the relationship between the number of CTCs subgroups and disease characteristics.