虽然内生真菌为增强植物对非生物胁迫的抵抗力提供了有希望的途径,这种生物强化背后的分子机制在很大程度上仍然未知。这项研究采用了多方面的方法,结合植物生理学,蛋白质组学,代谢组学,和有针对性的激素分析,以阐明甘蓝型油菜在相互作用的新生阶段对交替顶铵盐的早期反应。值得注意的是,在最佳生长条件下,对真菌的最初反应相对微妙,植物表型没有明显的改变,对蛋白质组和代谢组的影响很小。有趣的是,鉴定出的与根顶铵反应相关的蛋白质包括TUDOR1、膜联蛋白D4和质体K+外排反转运蛋白,暗示可能对抗非生物压力的潜在过程,特别是盐胁迫。随后的实验验证了这一假设,显示在盐胁迫下接种了根尖铵的植物的生长显着增强。分子分析揭示了对植物蛋白质组的深远的影响,在接种的植物中,超过50%的盐应激反应蛋白未受影响。顶铵调节的核糖体蛋白,光合蛋白质的丰度增加,增强ROS代谢,V-ATP酶的积累,改变了各种代谢酶的丰度,并可能促进脱落酸信号传导。随后的分析验证了这种激素的积累及其增强的信号传导。总的来说,这些发现表明,通过协调脱落酸信号促进耐盐性,启动植物的抗氧化系统,正如ROS清除代谢物的积累和ROS代谢的改变所证明的那样,导致ROS水平降低和光合作用增强。此外,它通过V-ATP酶积累调节离子固存,可能导致观察到的氯化物含量下降。
While endophytic fungi offer promising avenues for bolstering plant resilience against abiotic stressors, the molecular mechanisms behind this biofortification remain largely unknown. This study employed a multifaceted approach, combining plant physiology, proteomic, metabolomic, and targeted hormonal analyses to illuminate the early response of Brassica napus to
Acremonium alternatum during the nascent stages of their interaction. Notably, under optimal growth conditions, the initial reaction to fungus was relatively subtle, with no visible alterations in plant phenotype and only minor impacts on the proteome and metabolome. Interestingly, the identified proteins associated with the
Acremonium response included TUDOR 1, Annexin D4, and a plastidic K+ efflux antiporter, hinting at potential processes that could counter abiotic stressors, particularly salt stress. Subsequent experiments validated this hypothesis, showcasing significantly enhanced growth in
Acremonium-inoculated plants under salt stress. Molecular analyses revealed a profound impact on the plant\'s proteome, with over 50% of salt stress response proteins remaining unaffected in inoculated plants.
Acremonium modulated ribosomal proteins, increased abundance of photosynthetic proteins, enhanced ROS metabolism, accumulation of V-ATPase, altered abundances of various metabolic enzymes, and possibly promoted abscisic acid signaling. Subsequent analyses validated the accumulation of this hormone and its enhanced signaling. Collectively, these findings indicate that
Acremonium promotes salt tolerance by orchestrating abscisic acid signaling, priming the plant\'s antioxidant system, as evidenced by the accumulation of ROS-scavenging metabolites and alterations in ROS metabolism, leading to lowered ROS levels and enhanced photosynthesis. Additionally, it modulates ion sequestration through V-ATPase accumulation, potentially contributing to the observed decrease in chloride content.