移植后的存活率降低限制了干细胞的临床应用,因此本研究探讨了预处理脂肪干细胞(ADMSCs)和全反式维甲酸(ATRA)对引起急性肾损伤(AKI)的顺铂的影响。将150只雄性SD大鼠分为5组:对照组;顺铂(CIS)组;CIS和ATRA组;CIS和AMSC组,和CIS,ATRA,和ADMSCs组。十只大鼠在第3次之后被安乐死,Seven,注射CIS后的第11天。肾功能,分子研究,并进行了组织病理学分析。用ATRA预处理ADMSCs增加了细胞的活力,这反映在通过显着降低血清肌酐来改善CIS注射后的肾功能。微量白蛋白尿,以及不,肌酐清除率显著上升,以及SOD与顺铂组相比。与逆转顺铂作用的顺铂组相比,ATRA还通过显著下调半胱天冬酶-3、il-6和TGFβ1以及显著上调HIF1、VEGF和CD31来支持ADMSCs。ATRA通过减少细胞凋亡增加ADMSCs对顺铂诱导的AKI的肾脏保护特性,炎症,刺激血管生成。
The survival reduction after transplantation limited the clinical uses of stem cells so the current study explored preconditioning adipose-derived stem cells (ADMSCs) and all-trans retinoic acid (ATRA) effects on cisplatin that caused acute kidney injury (AKI). One hundred and fifty Sprague-Dawley male rats were distributed into five groups: control group; Cisplatin (CIS) group; CIS and ATRA group; CIS and ADMSC group, and CIS, ATRA, and ADMSCs group. Ten rats were euthanized after 3rd, 7th, and 11th days from CIS injection. Renal function, molecular studies, and histopathological analysis were studied. The preconditioning of ADMSCs with ATRA increased the viability of the cells which was reflected in the amelioration of kidney functions after CIS injection by the significant reduction of serum creatinine, microalbuminuria, as well as NO, and the significant rise of creatinine clearance, as well as SOD compared to the group of cisplatin. ATRA also supported ADMSCs by a significant down-regulation of caspase-3, il-6 and TGFβ1, and a significant up-regulation of HIF1, VEGF and CD31 compared to group of cisplatin which reversed the cisplatin effect. ATRA increased renoprotective properties of ADMSCs against cisplatin- induced AKI by reducing the apoptosis, inflammation, and stimulating angiogenesis.