目的:本研究评估了三种不同根管封闭剂的生物相容性(Well-RootSt,GuttaFlowBioseal,和AH-Plus)植入大鼠皮下组织后,使用组织病理学免疫组织化学分析。
方法:四组八十四只雄性大鼠,每组进行聚乙烯管背侧皮下植入,要么是空的,要么是满的。组织被收集,固定,并在7、15和30d后进行组织学分析。对载玻片拍照并进行数字处理,以使用分化簇3(CD3)和分化簇68(CD68)标记物鉴定淋巴细胞和巨噬细胞。分别。当使用单向方差分析(ANOVA)在组间和观察时间比较淋巴细胞和巨噬细胞浸润时,P设为0.05。
结果:所有组的组织病理学分析显示,7天后出现炎症反应,随后出现纤维囊。30天后,纤维囊的厚度和炎症反应消退。免疫组织化学分析的CD3染色显示,AH-Plus组在第7天和第15天淋巴细胞浸润的平均百分比最高,紧随其后的是Well-RootSt,GuttaFlowBioseal,和控制组。30天后,在淋巴细胞浸润的平均百分比方面,两组之间没有观察到明显的差异。在7、15和30天后,各组巨噬细胞浸润的平均百分比有显著差异,如CD68染色所示。在7、15和30天后,AH-Plus组的巨噬细胞浸润平均百分比最高,其次是Well-RootSt.和GuttaFlowBioseal小组,而对照组的平均百分比最低。
结论:所有观察期均显示对GuttaFlowBioseal的炎症反应最小。在大鼠模型中植入皮下组织后,到第30天,Well-RootSt和AH-Plus的初始炎症反应已经减轻,所有测试的封闭剂都具有出色的生物相容性.
This study was assessed the biocompatibility of three different root canal sealers (Well-Root St, GuttaFlow Bioseal, and AH-Plus) following implantation in rat subcutaneous tissues, using histopathological immunohistochemical analysis.
Four groups of eighty-four male rats each underwent subcutaneous dorsal implantation of a polyethylene tube, either empty or filled. Tissues were collected, fixed, and processed for histological analysis after 7, 15, and 30 d. Slides were photographed and digitally processed to identify lymphocytes and macrophages using Cluster of differentiation 3 (CD3) and cluster of differentiation 68 (CD68) markers, respectively. P was set at 0.05, when lymphocyte and macrophage infiltration was compared between groups and observation times using one-way analysis of variance (ANOVA).
Histopathological analysis of all groups revealed an inflammatory reaction followed by the emergence of a fibrous capsule after 7 days. After 30 days, the thickness of the fibrous capsule and the inflammatory response subsided. CD3 staining for immunohistochemical analysis revealed that the AH-Plus group had the highest mean percentage of lymphocyte infiltration at 7 and 15 days, followed by the Well-Root St, GuttaFlow Bioseal, and Control groups. After 30 days, no discernible difference was observed between the groups in terms of the mean percentage of lymphocyte infiltration. After 7, 15, and 30 days, there was a significant difference in the mean percentage of macrophage infiltration across the groups, as demonstrated by CD68 staining. After 7, 15, and 30 days, the AH-Plus group had the highest mean percentage of macrophage infiltration, followed by the Well-Root St. and GuttaFlow Bioseal groups, while the control group had the lowest mean percentage.
All observational periods showed minimal inflammatory reactions to GuttaFlow Bioseal. After subcutaneous tissue implantation in a rat model, the initial inflammatory reactions to Well-Root St and AH-Plus had abated by day 30, and all tested sealers had outstanding biocompatibility.