PDF(Pubmed)
Abstract：
UNASSIGNED: The objective of the study was to compare and evaluate the depth of penetration of an epoxy resin-based sealer following a final rinse of 17% ethylenediaminetetraacetic acid (EDTA) and 18% 1-hydroxyethylidene 1, 1-diphosphonate (HEDP), with diode laser and passive ultrasonic activation (PUI): an in vitro confocal laser scanning microscopy study.
UNASSIGNED: Fifty-two extracted human mandibular premolar teeth with single root and single canal were selected. They were disinfected in 0.1% thymol solution, cleaned of calculus and soft tissues, and stored in 0.1% thymol solution till use. All teeth were radiographed and selected as per the inclusion and exclusion criteria. The teeth were decoronated using a diamond disk under copious water spray to acquire a standardized root length of 14 mm. Working length was established by inserting a size 10-K file into each root canal until it is visible at the apical foramen and by subtracting 1 mm from the recorded length. Instrumentation of the root canal was done till master apical file size of F3 using ProTaper universal, rotary instruments. The canals were irrigated with 2 mL of 3% sodium hypochlorite between successive files. Teeth were randomly divided into four subgroups n = 12 according to the intervention. Passive ultrasonic irrigation and diode laser were used to activate the irrigants. Final irrigation was performed with distilled water. These specimens were examined using confocal laser scanning microscope (OLYMPUS FLUOVIEW FV 3000) for dentinal tubule penetration of the sealer. Two-way ANOVA test and Tukey\'s multiple post hoc test were used for statistical analysis.
UNASSIGNED: Highly significant difference was seen between the groups with EDTA and HEDP, with HEDP demonstrating the highest penetration. Among the activation techniques used in this study, PUI showed the highest penetration of the sealer. The least penetration was seen with diode laser activation and EDTA.
UNASSIGNED: The irrigation activation techniques significantly influence the penetration of sealer into root dentinal tubules. When penetration of sealer with different irrigation techniques and irrigants was evaluated, significant greater level of sealer penetration was attained with PUI activation of HEDP.
UNASSIGNED: Fifty-two extracted human mandibular premolar teeth with single root and single canal were selected. They were disinfected in 0.1% thymol solution, cleaned of calculus and soft tissues, and stored in 0.1% thymol solution till use. All teeth were radiographed and selected as per the inclusion and exclusion criteria. The teeth were decoronated using a diamond disk under copious water spray to acquire a standardized root length of 14 mm. Working length was established by inserting a size 10-K file into each root canal until it is visible at the apical foramen and by subtracting 1 mm from the recorded length. Instrumentation of the root canal was done till master apical file size of F3 using ProTaper universal, rotary instruments. The canals were irrigated with 2 mL of 3% sodium hypochlorite between successive files. Teeth were randomly divided into four subgroups n = 12 according to the intervention. Passive ultrasonic irrigation and diode laser were used to activate the irrigants. Final irrigation was performed with distilled water. These specimens were examined using confocal laser scanning microscope (OLYMPUS FLUOVIEW FV 3000) for dentinal tubule penetration of the sealer. Two-way ANOVA test and Tukey\'s multiple post hoc test were used for statistical analysis.
UNASSIGNED: Highly significant difference was seen between the groups with EDTA and HEDP, with HEDP demonstrating the highest penetration. Among the activation techniques used in this study, PUI showed the highest penetration of the sealer. The least penetration was seen with diode laser activation and EDTA.
UNASSIGNED: The irrigation activation techniques significantly influence the penetration of sealer into root dentinal tubules. When penetration of sealer with different irrigation techniques and irrigants was evaluated, significant greater level of sealer penetration was attained with PUI activation of HEDP.
摘要：
■该研究的目的是比较和评估环氧树脂基密封剂在17％乙二胺四乙酸（EDTA）和18％1-羟基亚乙基1，1-二膦酸盐（HEDP）的最终冲洗后的渗透深度，二极管激光和被动超声激活（PUI）：体外共聚焦激光扫描显微镜研究。
■选择52颗单根单管的人下颌前磨牙。用0.1%百里酚溶液消毒,清除结石和软组织，并保存在0.1%百里酚溶液中直至使用。根据纳入和排除标准对所有牙齿进行放射照相和选择。在大量喷水下使用金刚石圆盘装饰牙齿，以获得14毫米的标准化根部长度。通过将大小为10-K的文件插入每个根管直到在根尖孔处可见，并从记录的长度中减去1毫米来确定工作长度。使用ProTaper通用进行根管的仪器直到主根尖文件大小为F3，旋转仪器。在连续的文件之间用2mL的3%次氯酸钠灌溉运河。根据干预措施，将牙齿随机分为四个亚组，n=12。被动超声冲洗和二极管激光用于激活冲洗剂。最后用蒸馏水进行灌溉。使用共聚焦激光扫描显微镜（OLYMPUSFLUOVIEWFV3000）检查这些标本，以检查密封剂的牙本质小管渗透。采用双因素方差分析和Tukey的多重事后检验进行统计分析。
■在使用EDTA和HEDP的组之间观察到非常显著的差异,HEDP表现出最高的渗透率。在这项研究中使用的激活技术中，PUI显示封口机的穿透性最高。用二极管激光激活和EDTA观察到最少的穿透。
■灌溉活化技术显着影响封闭剂向根牙本质小管的渗透。当评估不同灌溉技术和灌溉剂对密封剂的渗透性时，通过HEDP的PUI活化获得了更高的密封剂渗透水平。
■选择52颗单根单管的人下颌前磨牙。用0.1%百里酚溶液消毒,清除结石和软组织，并保存在0.1%百里酚溶液中直至使用。根据纳入和排除标准对所有牙齿进行放射照相和选择。在大量喷水下使用金刚石圆盘装饰牙齿，以获得14毫米的标准化根部长度。通过将大小为10-K的文件插入每个根管直到在根尖孔处可见，并从记录的长度中减去1毫米来确定工作长度。使用ProTaper通用进行根管的仪器直到主根尖文件大小为F3，旋转仪器。在连续的文件之间用2mL的3%次氯酸钠灌溉运河。根据干预措施，将牙齿随机分为四个亚组，n=12。被动超声冲洗和二极管激光用于激活冲洗剂。最后用蒸馏水进行灌溉。使用共聚焦激光扫描显微镜（OLYMPUSFLUOVIEWFV3000）检查这些标本，以检查密封剂的牙本质小管渗透。采用双因素方差分析和Tukey的多重事后检验进行统计分析。
■在使用EDTA和HEDP的组之间观察到非常显著的差异,HEDP表现出最高的渗透率。在这项研究中使用的激活技术中，PUI显示封口机的穿透性最高。用二极管激光激活和EDTA观察到最少的穿透。
■灌溉活化技术显着影响封闭剂向根牙本质小管的渗透。当评估不同灌溉技术和灌溉剂对密封剂的渗透性时，通过HEDP的PUI活化获得了更高的密封剂渗透水平。
