以前发现,与香烟烟雾相比,加热烟草制品产生的气溶胶含有较少和较低的有害和潜在有害成分(HPHCs),在体外模型中引起较低的生物活性,在临床研究中引起较低的吸烟相关暴露生物标志物水平.重要的是要积累这样的科学证据加热烟草产品与一个新的加热系统,因为不同的加热系统可能会影响所产生的气溶胶的HPHC的量的定量方面和生物活性的定性方面。这里,的化学性质,和对DT3.0a排放的气溶胶的毒理学反应,具有新型加热系统的新型加热烟草产品,和香烟烟雾(CS)进行了比较,使用化学分析,体外电池(标准化遗传毒性和细胞毒性)测定,和机械(ToxTracker和二维细胞培养)测定。测试了regular和薄荷醇风味的DT3.0a和标准1R6F参考香烟。DT3.0a气溶胶中选定的HPHC产率低于1R6FCS。基因毒性相关的测定表明DT3.0a气雾剂没有基因毒性,不管代谢激活。其他生物学分析表明,与1R6FCS相比,DT3.0a气溶胶引起的细胞毒性诱导和氧化应激反应较少。对于普通和薄荷醇DT3.0a都发现了类似的结果。与以前关于使用其他加热系统加热烟草产品的报告一样,这项研究的结果表明,与1R6FCS相比,DT3.0a气溶胶具有较小的化学和生物学特性。
It has previously been found that, compared with cigarette smoke, the aerosols generated by heated tobacco products contain fewer and lower harmful and potentially harmful constituents (HPHCs) and elicit lower biological activity in in vitro models and lower smoking-related exposure biomarker levels in clinical studies. It is important to accumulate such scientific evidences for heated tobacco products with a novel heating system, because different heating system may affect the quantitative aspect of the amount of HPHCs and the qualitative aspect of the biological activity of the aerosol generated. Here, the chemical properties of, and toxicological responses to aerosols emitted by DT3.0a, a new heated tobacco product with a novel heating system, and cigarette smoke (CS) were compared, using chemical analyses, in vitro battery (standardized genotoxicity and cytotoxicity) assays, and mechanistic (ToxTracker and two-dimensional cell culture) assays. Regular- and menthol-flavored DT3.0a and standard 1R6F reference cigarettes were tested. Selected HPHC yields were lower in DT3.0a aerosol than 1R6F CS. The genotoxicity-related assays indicated that DT3.0a aerosol was not genotoxic, regardless of metabolic activation. The other biological assays indicated that less cytotoxicity induction and oxidative stress response were elicited by DT3.0a aerosol compared with 1R6F CS. Similar results were found for both regular and menthol DT3.0a. Like previous reports for heated tobacco products with other heating systems, the results of this study indicated that DT3.0a aerosols have chemical and biological properties less likely to be harmful than 1R6F CS.