目的:开发和验证一个简单的,准确的HPTLC方法分析8-姜酚和测定生姜提取物和含生姜的膳食补充剂中8-姜酚的量,茶和商业面霜。
方法:在涂有0.2mm硅胶60F254层的10×20cm铝衬板上进行分析(E-Merck,德国)以正己烷:乙酸乙酯60:40(v/v)为流动相。CamagTLC扫描仪III用于569处的UV光密度扫描。
结果:发现该系统在保留因子(Rf)值为(0.39±0.04)时产生8-姜酚的致密斑点,线性范围为50-500ng/点(r(2)=0.9987)。检测限(12.76纳克/点),定量限(26.32纳克/点),准确度(小于2%)和回收率(范围从98.22-99.20)被发现令人满意。
结论:发现用于定量8-姜酚的HPTLC方法简单,准确,可重复,敏感,适用于分析生姜提取物和含生姜的膳食补充剂中的8-姜酚,茶和商业面霜。
OBJECTIVE: To develop and validate a simple, accurate HPTLC method for the analysis of 8-gingerol and to determine the quantity of 8-gingerol in Zingiber officinale extract and ginger-containing dietary supplements, teas and commercial creams.
METHODS: The analysis was performed on 10×20 cm aluminium-backed plates coated with 0.2 mm layers of silica gel 60 F254 (E-Merck, Germany) with n-hexane: ethyl acetate 60: 40 (v/v) as mobile phase. Camag TLC Scanner III was used for the UV densitometric scanning at 569.
RESULTS: This system was found to give a compact spot of 8-gingerol at retention factor (Rf) value of (0.39±0.04) and linearity was found in the ranges 50-500 ng/spot (r (2)=0.9987). Limit of detection (12.76 ng/spot), limit of quantification (26.32 ng/spot), accuracy (less than 2 %) and recovery (ranging from 98.22-99.20) were found satisfactory.
CONCLUSIONS: The HPTLC method developed for quantification of 8-gingerol was found to be simple, accurate, reproducible, sensitive and is applicable to the analysis of 8-gingerol in Zingiber officinale extract and ginger-containing dietary supplements, teas and commercial creams.