从异叶树的根中分离出两种异戊二烯化的2-芳基苯并呋喃,结合各种色谱方法,包括ODS,MCI、SephadexLH-20和半制备型高效液相色谱(HPLC)。它们被鉴定为5-[6-羟基-4-甲氧基-5,7-双(3-甲基丁-2-烯基)苯并呋喃-2-基]-1,3-苯二酚(1)和5-[2H,9H-2,2,9,9-四甲基-呋喃并[2,3-f]吡喃并[2,3-h][1]苯并吡喃-6-基]-1,3-苯二酚(2)如HR-ESI-MS,IR,1DNMR,和2DNMR,并命名为树胶蛋白B(1)和C(2),分别。用佛波醇12-肉豆蔻酸酯13-乙酸酯(PMA)刺激的大鼠多形核中性粒细胞(PMN)评估了两种化合物的抗呼吸爆发活性。结果表明,1和2对PMN的呼吸爆发具有明显的抑制作用,IC_(50)值为0.27和1.53μmol·L〜(-1),分别。
Two prenylated 2-arylbenzofurans were isolated from roots of Artocarpus heterophyllus, with a combination of various chromatographic approaches, including ODS, MCI, Sephadex LH-20, and semipreparative high performance liquid chromatography(HPLC). They were identified as 5-[6-hydroxy-4-methoxy-5,7-bis(3-methylbut-2-enyl)benzofuran-2-yl]-1,3-benzenediol(1) and 5-[2H,9H-2,2,9,9-tetramethyl-furo[2,3-f]pyrano[2,3-h][1]benzopyran-6-yl]-1,3-benzenediol(2) with spectroscopic methods, such as HR-ESI-MS, IR, 1D NMR, and 2D NMR, and named artoheterins B(1) and C(2), respectively. The anti-respiratory burst activities of the two compounds were evaluated with rat polymorphonuclear neutrophils(PMNs) stimulated by phorbol 12-myristate 13-acetate(PMA). The results showed that 1 and 2 exhibited significant inhibitory effect on respiratory burst of PMNs with IC_(50) values of 0.27 and 1.53 μmol·L~(-1), respectively.