大黄(RR),中文名大黄,通常用于缺血性中风(IS)的治疗。然而,其潜在机制尚未完全阐明。本研究旨在验证RR对IS的影响,并探讨RR预防IS的可能机制。通过栓塞性大脑中动脉阻塞(MCAO)手术诱导雄性大鼠IS,手术前半小时给药。RR显著降低了神经功能缺损评分,脑梗死体积,和脑水肿率,改善MCAO大鼠脑组织局部脑血流量(rCBF)及组织病理学改变。16SrRNA分析显示,有害微生物如Fournierella和Bilophila减少,和有益的微生物,如肠道,去氟科,Christensenellaceae,和Lachnospira显著增加,RR预处理后。1H-核磁共振(1H-NMR)用于检测血清代谢组学,和RR治疗显著改变了代谢物的水平,如异亮氨酸,缬氨酸,N6-乙酰赖氨酸,蛋氨酸,3-氨基异丁酸,N,N-二甲基甘氨酸,丙二醇,三甲胺N-氧化物,肌醇,胆碱,甜菜碱,乳酸,葡萄糖,和脂质,差异代谢产物的富集分析表明,RR可能参与氨基酸代谢和能量代谢的调节。RR通过调节肠道细菌和代谢途径发挥抗IS的作用。
Rhubarb (RR), Chinese name Dahuang, is commonly used in the treatment of ischemic stroke (IS). However, its potential mechanism is not fully elucidated. This study intended to verify the effect of RR on IS and investigate the possible mechanism of RR in preventing IS. IS in male rats was induced by embolic middle cerebral artery occlusion (MCAO) surgery, and drug administration was applied half an hour before surgery. RR dramatically decreased the neurological deficit scores, the cerebral infarct volume, and the cerebral edema rate, and improved the regional cerebral blood flow (rCBF) and histopathological changes in the brain of MCAO rats. The 16S rRNA analysis showed the harmful microbes such as Fournierella and Bilophila were decreased, and the beneficial microbes such as Enterorhabdus, Defluviitaleaceae, Christensenellaceae, and Lachnospira were significantly increased, after RR pretreatment. 1H-nuclear magnetic resonance (1H-NMR) was used to detect serum metabolomics, and RR treatment significantly changed the levels of metabolites such as isoleucine, valine, N6-acetyllysine, methionine, 3-aminoisobutyric acid, N, N-dimethylglycine, propylene glycol, trimethylamine N-oxide, myo-inositol, choline, betaine, lactate, glucose, and lipid, and the enrichment analysis of differential metabolites showed that RR may participate in the regulation of amino acid metabolism and energy metabolism. RR exerts the role of anti-IS via regulating gut bacteria and metabolic pathways.