PDF(Pubmed)
Abstract：
Due to the increasing populations of anthelmintic-resistant gastrointestinal nematodes and as a consequence of the adverse effects of synthetic drugs, this study focuses on the search for secondary metabolites with nematocidal activity from the edible mushroom Pleurotus djamor using The proton nuclear magnetic resonance (1H-NMR) metabolomics. The highest activity was shown by the ethyl acetate fractions of mycelium (EC50 290.8 µg/mL) and basidiomes (EC50 282.7 µg/mL). Principal component analysis (PCA) and hierarchical data analysis (HCA) of the 1H-NMR metabolic profiles data showed that the ethanolic extracts, the ethyl acetate, butanol, and water fractions from mycelium have different metabolic profiles than those from basidiomes, while low polarity (hexane) fractions from both stages of fungal development show similar profiles. Orthogonal partial least squares discriminant analysis (OPLS-DA) allowed the identification of signals in the 1H-NMR metabolic profile associated with nematocidal activity. The signals yielded via OPLS-DA and bidimensional NMR analysis allowed the identification of uracil as a component in the ethyl acetate fraction from basidiomes, with an EC50 of 237.7 µg/mL. The results obtained showed that chemometric analyses of the 1H-NMR metabolic profiles represent a viable strategy for the identification of bioactive compounds from samples with complex chemical profiles.
摘要：
由于抗驱虫药的胃肠道线虫数量不断增加，并且由于合成药物的不良反应，这项研究的重点是使用质子核磁共振（1H-NMR）代谢组学从食用蘑菇侧耳中寻找具有杀线虫活性的次生代谢产物。菌丝体（EC50290.8µg/mL）和菌丝（EC50282.7µg/mL）的乙酸乙酯级分显示出最高的活性。1H-NMR代谢谱数据的主成分分析(PCA)和分层数据分析(HCA)显示,乙酸乙酯，丁醇,和来自菌丝体的水部分有不同的代谢谱，而来自两个真菌发展阶段的低极性（己烷）级分显示相似的概况。正交偏最小二乘判别分析（OPLS-DA）可以鉴定与杀线虫活性相关的1H-NMR代谢谱中的信号。通过OPLS-DA和二维NMR分析产生的信号允许鉴定尿嘧啶作为来自碱茎的乙酸乙酯部分中的成分，EC50为237.7µg/mL。获得的结果表明，1H-NMR代谢谱的化学计量学分析代表了从具有复杂化学谱的样品中鉴定生物活性化合物的可行策略。
