目的:这项工作的目的是优化可印刷的聚己内酯(PCL)/β-磷酸三钙(β-TCP)生物材料,其具有高百分比的β-TCP具有平衡的机械特性,类似于人类松质骨,推测是改善成骨。
方法:PCL/β-TCP支架是从定制的长丝获得的,用于熔融沉积建模(FDM)3D打印,其中β-TCP的量增加。样品机械特征,表面形貌和润湿性进行了评估,以及细胞相容性测定,细胞粘附和分化。
结果:新制造的材料的参数对于PCL/β-TCP支架制造是最佳的。与对照相比,复合材料表面显示出更高的亲水性,它们的表面粗糙度急剧上升,可能是由于β-TCP的存在。复合材料的杨氏模量明显高于原始PCL,表明β-TCP的固有强度有利于提高复合生物材料的弹性模量。与PCL对照相比,所有新型复合生物材料都支持更大的细胞生长和更强的成骨细胞分化。
结论:该项目突出了制造,通过FDM无溶剂方法,高达70%浓度的β-TCP的PCL/β-TCP支架。克服了文献中目前60%的lmit。3D打印和定制生物材料的结合允许生产高度个性化的支架,具有类似于自然结构和骨骼组成的最佳机械和生物学特征。这强调了这种结构有望用于骨和牙周再生的创新方法。
OBJECTIVE: The purpose of this work was to optimise printable polycaprolactone (PCL)/β-tricalcium phosphate (β-TCP) biomaterials with high percentages of β-TCP endowed with balanced mechanical characteristics to resemble human cancellous bone, presumably improving osteogenesis.
METHODS: PCL/β-TCP scaffolds were obtained from customised filaments for fused deposition modelling (FDM) 3D printing with increasing amounts of β-TCP. Samples mechanical features, surface topography and wettability were evaluated as well as cytocompatibility assays, cell adhesion and differentiation.
RESULTS: The parameters of the newly fabricated materila were optimal for PCL/β-TCP scaffold fabrication. Composite surfaces showed higher hydrophilicity compared with the controls, and their surface roughness sharply was higher, possibly due to the presence of β-TCP. The Young\'s modulus of the composites was significantly higher than that of pristine PCL, indicating that the intrinsic strength of β-TCP is beneficial for enhancing the elastic modulus of the composite biomaterials. All novel composite biomaterials supported greater cellular growth and stronger osteoblastic differentiation compared with the PCL control.
CONCLUSIONS: This project highlights the possibility to fabricat, through an FDM solvent-free approach, PCL/β-TCP scaffolds of up to 70 % concentrations of β-TCP. overcoming the current lmit of 60 % stated in the literature. The combination of 3D printing and customised biomaterials allowed production of highly personalised scaffolds with optimal mechanical and biological features resembling the natural structure and the composition of bone. This underlines the promise of such structures for innovative approaches for bone and periodontal regeneration.