研究表明,维生素E乙酸酯(VEA),当用于电子烟装置时,经历热降解,被认为是电子烟或Vaping产品使用相关肺损伤(EVALI)的主要贡献者之一。使用Borgwaldt5.1线性吸烟者,SVS250电子蒸发器,和两种类型的坦克系统,当使用乙腈提取含有蒸发的VEA的过滤器时,分析VEA通过烟草科学研究合作中心(CORESTA)方法81产生的降解产物。确定的两种主要产物是2,3,5,6-四甲基-1,4-苯醌和2,6,10,14-四甲基-1-十五碳烯,使用分析标准和气相色谱-高分辨率质谱(GC-HRMS)进行了确认。4-乙酰氧基-2,3,5,6-四甲基-2,4-环己二烯酮的额外合成和随后使用核磁共振(NMR)和GC-HRMS的表征表明,这不是产生的产物之一。这些降解物的鉴定将允许未来的研究量化和检查体内和体外降解物作为暴露和毒性评估的生物标志物。
Studies have suggested that vitamin E acetate (VEA), when used in an electronic vaping device, undergoes thermal degradation and is considered one of the main contributors in e-cigarette or vaping product use-associated lung injury (EVALI). Using a Borgwaldt 5.1 linear smoker, a SVS250 Electronic Vaporizer and two types of tank systems, VEA was analyzed for degradation products produced via the Cooperation Centre for Scientific Research Relative to Tobacco method 81 when the filter containing vaporized VEA was extracted using acetonitrile. Two of the major products identified were 2,3,5,6-tetramethyl-1,4-benzoquinone and 2,6,10,14-tetramethyl-1-pentadecene, which were confirmed using analytical standards and gas chromatography-high-resolution mass spectrometry (GC-HRMS). Additional synthesis of 4-acetoxy-2,3,5,6-tetramethyl-2,4-cyclohexadienone and subsequent characterization using nuclear magnetic resonance and GC-HRMS suggested that this is not one of the products produced. Identification of these degradants will allow future studies to quantify and examine the degradants in vivo and in vitro as biomarkers for exposure and toxicity assessment.