■本研究旨在确定右桂丸与五子岩宗丸(DYWWYW)的联合作用和机理,一种传统的中草药配方,在95%乙醇诱导的子宫内膜薄型小鼠模型中。
■薄型子宫内膜小鼠用孕酮(0.002mg)以及低剂量和高剂量的DYWWYW(0.05和0.5mLDYWWYW,分别,在2mL生理盐水中稀释)。进行蛋白质印迹和qRT-PCR分析以确定蛋白质和mRNA表达水平,分别,子宫组织中整合素αγβ3和白血病抑制因子(LIF)的表达。通过ELISA测定血清雌二醇和孕酮浓度。其余薄型子宫内膜小鼠与雄性小鼠交配,并计算不同组植入的胚胎数量。
■高剂量的DYWWYW可有效改善95%乙醇引起的子宫内膜损伤。雌二醇的水平,黄体酮,用高剂量DYWWYW治疗的薄型子宫内膜小鼠中的αγβ3和LIF也显着升高。此外,高剂量的DYWWYW可显着增加子宫内膜薄型小鼠的胚胎植入次数。
■DYWWYW通过提高内源性雌二醇水平对薄型子宫内膜有改善作用,黄体酮,小鼠模型中的αγβ3和LIF。
在生殖周期中,子宫内膜厚度超过7mm被认为是成功胚胎植入的临界值.目前,虽然治疗子宫内膜厚度的改善,如西地那非,子宫内膜刮除,已经尝试了粒细胞集落刺激因子和低剂量的阿司匹林,对患者的影响并不一致。因此,有必要寻求新的治疗方法来有效增加子宫内膜厚度。本研究建立了95%乙醇诱导的子宫内膜薄型雌性ICR小鼠模型。高剂量右归丸汤联合五子延宗丸(DYWWYW)可有效改善子宫内膜损伤,并显着增加薄型子宫内膜小鼠的胚胎植入次数。此外,包括雌二醇在内的一些关键指标的水平,黄体酮,在用高剂量DYWWYW处理的薄型子宫内膜小鼠中,αγβ3和LIF也增加。因此,DYWWYW在小鼠模型中增加子宫内膜厚度方面是可行的。
UNASSIGNED: This study aimed to determine the effect and mechanism of the Decoction of Yougui Wan combined with Wuzi Yanzong Wan (DYWWYW), a traditional Chinese herbal formula, in a mouse model with thin endometrium induced by 95%
ethanol.
UNASSIGNED: Thin endometrium mice were treated with progynova (0.002 mg) as well as a low and high dose of DYWWYW (0.05 and 0.5 mL DYWWYW, respectively, diluted in 2 mL normal saline). Western blotting and qRT-PCR analyses were performed to determine the protein and mRNA expression levels, respectively, of integrin αγβ3 and leukaemia inhibitor factor (LIF) in uterus tissues. Serum oestradiol and progesterone concentrations were determined via ELISA. The remaining thin endometrium mice were mated with male mice, and the number of embryos implanted in the different groups was calculated.
UNASSIGNED: A high dose of DYWWYW effectively ameliorated the injury of endometrium caused by 95%
ethanol. The levels of oestradiol, progesterone, αγβ3 and LIF in thin endometrium mice treated with a high dose of DYWWYW were also significantly elevated. Additionally, a high dose of DYWWYW remarkably increased the number of embryo implantations in mice with thin endometrium.
UNASSIGNED: DYWWYW has improvement effects on thin endometrium by elevating the levels of endogenous oestradiol, progesterone, αγβ3, and LIF in a mouse model.
During the reproductive cycle, endometrium thickness of more than 7 mm is considered as a cut-off value for successful embryo implantation. Currently, although therapies for the improvement of endometrium thickness such as sildenafil, endometrial scraping, granulocyte colony-stimulating factor and low dose of aspirin have been tried, the effects on patients are not consistent. Consequently, it is necessary to seek novel therapies to increase endometrium thickness effectively. A 95%
ethanol-induced thin endometrium female ICR mouse model was established in this study. High dose of Decoction of Yougui Wan combined with Wuzi Yanzong Wan (DYWWYW) effectively ameliorated the injury of endometrium and remarkably increased the number of embryo implantations in thin endometrium mice. Additionally, the levels of some key indicators including oestradiol, progesterone, αγβ3, and LIF were also increased in thin endometrium mice treated with high dose of DYWWYW. Therefore, DYWWYW was feasible in increasing endometrium thickness in a mouse model.