背景:长期饮酒会增加饮酒障碍的风险,导致各种神经系统疾病。然而,不同乙醇水平对慢性饮酒期间一系列行为的影响尚不清楚.在这项研究中,我们在两瓶选择(IA2BC)程序中建立了间歇性使用乙醇的方法,以探索乙醇对C57BL/6J小鼠行为表现的剂量依赖性影响。
方法:成年雄性C57BL/6J小鼠自愿获得不同浓度的乙醇(0%,5%,10%,和20%乙醇)在12周的IA2BC范例下。进行了一系列行为测试以评估疼痛阈值的变化,类似焦虑的行为,运动活动,电机协调,和认知。在每个会话期间监测乙醇消耗和偏好。此外,肝脏,心,肺组织采用病理显微镜检查。
结果:IA2BC范式下小鼠的平均(标准偏差)乙醇消耗剂量依赖性增加至5.1(0.2),8.7(0.7),15.9(0.8)g/kg/24h,含5%,10%,20%的乙醇,分别。然而,所有乙醇组的乙醇偏好没有显着差异。慢性饮酒导致痛觉过敏,认知障碍,和电机不协调,但没有引起体温的变化,运动活动,或者类似焦虑的行为。检测到肝脏的轻微组织病理学改变;然而,在心脏或肺部未观察到重大异常病理。
结论:这些发现阐明了12周IA2BC模型中乙醇剂量与小鼠行为变化之间的联系,从而弥合有关长期饮酒对神经系统疾病影响的知识差距。
BACKGROUND: Chronic alcohol drinking increases the risk of alcohol use disorders, causing various neurological disorders. However, the impact of different
ethanol levels on a spectrum of behaviors during chronic drinking remains unclear. In this study, we established an intermittent access to ethanol in a two-bottle choice (IA2BC) procedure to explore the dose-dependent effects of
ethanol on the behavioral performance of C57BL/6 J mice.
METHODS: Adult male C57BL/6 J mice were provided voluntary access to different ethanol concentrations (0 %, 5 %, 10 %, and 20 %
ethanol) under a 12-week IA2BC paradigm. A battery of behavioral tests was administered to assess alterations in pain threshold, anxiety-like behaviors, locomotor activity, motor coordination, and cognition.
Ethanol consumption and preference were monitored during each session. Moreover, the liver, heart, and lung tissues were examined using pathological microscopy.
RESULTS: The average (standard deviation) ethanol consumption of mice under the IA2BC paradigm increased dose-dependently to 5.1 (0.2), 8.7 (0.7), and 15.9 (0.8) g/kg/24 h with 5 %, 10 %, and 20 % ethanol, respectively. However, there is no significant difference in
ethanol preference among all the
ethanol groups. Chronic ethanol drinking caused hyperalgesia, cognitive impairment, and motor incoordination, but caused no changes in body temperature, locomotor activity, or anxiety-like behaviors. Minor histopathological alterations in the liver were detected; however, no major abnormal pathology was observed in the heart or lungs.
CONCLUSIONS: These findings clarify the link between ethanol dosage and behavioral changes in mice over a 12-week IA2BC paradigm, thereby bridging the knowledge gap regarding the effects of chronic ethanol drinking on neurological disorders.