急性胃肠炎(AGE)是一种常见的儿科感染,仍然是全球儿童发病和死亡的重要原因。特别是在低收入地区。因此,这项研究的目的是检测来自胃肠炎监测网的粪便样本中的人腺病毒(HAdV)和非脊髓灰质炎肠道病毒(NPEV),并通过核苷酸测序鉴定循环菌株。用qPCR/RT-qPCR检测了801份粪便样本,和657(82.0%)接种到HEp-2C和RD细胞系中。使用qPCR/RT-qPCR获得的HAdV和NPEV阳性率分别为31.7%(254/801)和10.5%(84/801),分别,5.4%(43/801)共检测。在9.6%(63/657)的患者中观察到细胞病变效应,2.7%(18/657)与HAdV相关,通过ICC-PCR检测后,与NPEV相关的为6.2%(41/657)。两种方法的比较表明,EVNP的一致性为93.5%,HAdV的一致性为64.4%。在整个研究期间检测到这两种病毒,HAdV阳性率从阿马帕州的41%到帕拉州的18%不等。NPV从帕拉/朗多尼亚的18%到英亩的3%不等。HAdV和NPEV受影响最大的年龄组均超过60个月。先前对轮状病毒和诺如病毒呈阳性的样本,在腹泻的存在或不存在方面没有显示出重大差异,发烧,呕吐,被排除在这两种病毒的临床分析之外。这些病毒传播了五年,缺席了几个月,主要是在与巴西SARS-CoV-2感染浪潮相对应的几个月内。鉴定出5种HAdV(A,B,C,D,andF),HAdV-F41(56.5%)占主导地位,其次是HAdV-C(15.2%)。三种NPEV(A,B,和C)被检测到,血清型E14(19.3%)和CVA-24(16.1%)是最普遍的。本研究表明,在巴西北部地区患有AGE症状的儿童中,NPEV和HAdV类型的多样性很高。
Acute gastroenteritis (AGE) is a common pediatric infection that remains a significant cause of childhood morbidity and mortality worldwide, especially in low-income regions. Thus, the objective of this study was to detect human adenovirus (HAdV) and non-polio
enterovirus (NPEV) in fecal samples from the Gastroenteritis Surveillance Network, and to identify circulating strains by nucleotide sequencing. A total of 801 fecal samples were tested using qPCR/RT-qPCR, and 657 (82.0%) were inoculated into HEp-2C and RD cell lines. The HAdV and NPEV positivity rates obtained using qPCR/RT-qPCR were 31.7% (254/801) and 10.5% (84/801), respectively, with 5.4% (43/801) co-detection. Cytopathic effect was observed in 9.6% (63/657) of patients, 2.7% (18/657) associated with HAdV, and 6.2% (41/657) associated with NPEV after testing by ICC-PCR. A comparison of the two methodologies demonstrated an agreement of 93.5% for EVNP and 64.4% for HAdV. These two viruses were detected throughout the study period, with HAdV positivity rates ranging from 41% in Amapá to 18% in Pará. The NEPV varied from 18% in Pará/Rondônia to 3% in Acre. The most affected age group was over 60 months for both HAdV and NPEV. Samples previously positive for rotavirus and norovirus, which did not show a major difference in the presence or absence of diarrhea, fever, and vomiting, were excluded from the clinical analyses of these two viruses. These viruses circulated over five years, with a few months of absence, mainly during the months corresponding to the waves of SARS-CoV-2 infection in Brazil. Five HAdV species were identified (A, B, C, D, and F), with a greater predominance of HAdV-F41 (56.5%) followed by HAdV-C (15.2%). Three NPEV species (A, B, and C) were detected, with serotypes E14 (19.3%) and CVA-24 (16.1%) being the most prevalent. The present study revealed a high diversity of NPEV and HAdV types circulating in children with AGE symptoms in the northern region of Brazil.