背景:生物技术提供了一种经济有效的方法来生产纳米材料,例如氧化银纳米颗粒(Ag2ONPs),它们已经成为具有不同应用的多功能实体。这项研究调查了内生细菌生物合成Ag2ONPs的能力。
结果:一种新的内生细菌菌株,烟子新杆菌AUMC-B524,分离自沙维枸杞。&Schult离开并用于在细胞外合成Ag2ONPS。采用Plackett-Burman设计和响应面方法优化Ag2ONPs(Bio-Ag2ONPs)的生物合成。综合表征技术,包括紫外可见光谱分析,傅里叶变换红外光谱,透射电子显微镜,X射线衍射,动态光散射分析,拉曼显微术,和能量色散X射线分析,确认了Ag2ONPS的精确组成。Bio-Ag2ONPs对多重耐药伤口病原体有效,最低抑制浓度(1-25µgmL-1)。值得注意的是,Bio-Ag2ONPs在体外对人皮肤成纤维细胞(HSF)无细胞毒性作用,在有效抑制人表皮样皮肤癌(A-431)细胞增殖的同时,诱导凋亡和调节关键凋亡基因,包括Bcl-2相关X蛋白(Bax),B细胞淋巴瘤2(Bcl-2),胱天蛋白酶-3(Cas-3),和基因组的监护人(P53)。
结论:这些发现突出了由内生N.niaciniAUMC-B524合成的Bio-Ag2ONPs的治疗潜力,强调了它们的抗菌功效,抗癌活性,和生物相容性,为新的治疗策略铺平道路。
BACKGROUND: Biotechnology provides a cost-effective way to produce nanomaterials such as silver oxide nanoparticles (Ag2ONPs), which have emerged as versatile entities with diverse applications. This study investigated the ability of endophytic bacteria to biosynthesize Ag2ONPs.
RESULTS: A novel endophytic bacterial strain, Neobacillus niacini AUMC-B524, was isolated from Lycium shawii Roem. & Schult leaves and used to synthesize Ag2ONPS extracellularly. Plackett-Burman design and response surface approach was carried out to optimize the biosynthesis of Ag2ONPs (Bio-Ag2ONPs). Comprehensive characterization techniques, including UV-vis spectral analysis, Fourier transform infrared spectroscopy, transmission electron microscopy, X-ray diffraction, dynamic light scattering analysis, Raman microscopy, and energy dispersive X-ray analysis, confirmed the precise composition of the Ag2ONPS. Bio-Ag2ONPs were effective against multidrug-resistant wound pathogens, with minimum inhibitory concentrations (1-25 µg mL-1). Notably, Bio-Ag2ONPs demonstrated no cytotoxic effects on human skin fibroblasts (HSF) in vitro, while effectively suppressing the proliferation of human epidermoid skin carcinoma (A-431) cells, inducing apoptosis and modulating the key apoptotic genes including Bcl-2 associated X protein (Bax), B-cell lymphoma 2 (Bcl-2), Caspase-3 (Cas-3), and guardian of the genome (P53).
CONCLUSIONS: These findings highlight the therapeutic potential of Bio-Ag2ONPs synthesized by endophytic N. niacini AUMC-B524, underscoring their antibacterial efficacy, anticancer activity, and biocompatibility, paving the way for novel therapeutic strategies.