Abstract：
The domestication process progressively differentiated wild relatives from modern cultivars, thus impacting plant-associated microorganisms. Endophytic bacterial communities play vital roles in plant growth, development, and health, which contribute to the crop\'s sustainable development. However, how plant domestication impacts endophytic bacterial communities and relevant root exudates in wheat remains unclear. First, we have observed that the domestication process increased the root endophytic microbial community diversity of wheat while decreasing functional diversity. Second, domestication decreased the endophytic bacterial co-occurrence network stability, and it did significantly alter the abundances of core microorganisms or potential probiotics. Third, untargeted LC-MS metabolomics revealed that domestication significantly altered the metabolite profiles, and the abundances of various root exudates released were significantly correlated with keystone taxa including the Chryseobacterium, Massilia, and Lechevalieria. Moreover, we found that root exudates, especially L-tyrosine promote the growth of plant-beneficial bacteria, such as Chryseobacterium. Additionally, with L-tyrosine and Chryseobacterium colonized in the roots, the growth of wild wheat\'s roots was significantly promoted, while no notable effect could be found in the domesticated cultivars. Overall, this study suggested that wild wheat as a key germplasm material, and its native endophytic microbes may serve as a resource for engineering crop microbiomes to improve the morphological and physiological traits of crops in widely distributed poor soils.
摘要：
驯化过程逐渐将野生近缘种与现代品种区分开来，从而影响与植物相关的微生物。内生细菌群落在植物生长中起着至关重要的作用,发展,和健康，这有助于作物的可持续发展。然而,植物驯化如何影响小麦中的内生细菌群落和相关根系分泌物尚不清楚。首先,我们观察到驯化过程增加了小麦根系内生微生物群落多样性，同时降低了功能多样性。第二,驯化降低了内生细菌共生网络的稳定性，它确实显著改变了核心微生物或潜在益生菌的丰度。第三，非靶向LC-MS代谢组学显示驯化显著改变了代谢物谱，释放的各种根系分泌物的丰度与包括金杆菌在内的梯形分类群显著相关，Massilia,和Lechevalieria.此外，我们发现根系分泌物，特别是L-酪氨酸促进植物有益细菌的生长，如金杆菌。此外,L-酪氨酸和金杆菌定植在根部，野生小麦的根系生长得到显著促进,而在驯化品种中没有发现显著的影响。总的来说,这项研究表明，野生小麦是关键种质材料，其原生内生微生物可作为工程作物微生物的资源，以改善分布广泛的贫瘠土壤中作物的形态和生理特性。
