纳米材料越来越多地用于生物医学成像和癌症治疗,如何提高细胞对纳米材料的内吞作用是一个关键问题。在此,对骨肉瘤细胞(MG-63)施加交流电(AC)电刺激可通过大胞吞作用使Fe3O4纳米颗粒(直径:50nm)的细胞内吞作用增加52.46%。这可以归因于F-肌动蛋白含量的降低和细胞内Ca2浓度的增加。透射电子显微镜,免疫荧光染色,westernblot,流式细胞术,电感耦合等离子体发射光谱仪的分析支持这种解释。电刺激的应用使磁热疗中的细胞活力降低了47.6%,磁共振成像的信号强度增加了29%。对于乳腺癌细胞(MCF-7)观察到类似的增强内吞作用,胶质母细胞瘤细胞(U-87MG),黑色素瘤细胞(A-375),和膀胱癌细胞(TCCSUP),还有直径为20和100纳米的Fe3O4纳米粒子,和直径为70nm的Zn0.54Co0.46Cr0.65Fe1.35O4纳米粒子。似乎电刺激有可能通过促进内吞作用来改善磁性纳米颗粒的诊断和治疗效果。
Nanomaterials are increasingly used in biomedical imaging and cancer therapy, and how to improve the
endocytosis of nanomaterials by cells is a key issue. The application of alternating current (AC) electrical stimulation to osteosarcoma cells (MG-63) here can increase the cellular
endocytosis of Fe3O4 nanoparticles (diameter: 50 nm) by 52.46% via macropinocytosis. This can be ascribed to the decrease in F-actin content and the increase in intracellular Ca2+ concentration. Transmission electron microscope, immunofluorescence staining, western blot, flow cytometry, and inductively coupled plasma emission spectrometer analyses support this interpretation. The application of electrical stimulation decreases the cell viability in magnetic hyperthermia by 47.6% and increases the signal intensity of magnetic resonance imaging by 29%. Similar enhanced
endocytosis is observed for breast cancer cells (MCF-7), glioblastoma cells (U-87 MG), melanoma cells (A-375), and bladder cancer cells (TCCSUP), and also for Fe3O4 nanoparticles with the diameters of 20 and 100 nm, and Zn0.54Co0.46Cr0.65Fe1.35O4 nanoparticles with the diameter of 70 nm. It seems the electrical stimulation has the potential to improve the diagnostic and therapeutic effects of magnetic nanoparticles by promoting
endocytosis.