双酚A(BPA)已广泛应用于聚碳酸酯(PC)塑料的生产,阻燃剂和环氧树脂,它是最重要的内分泌干扰物质之一,可对人体雌激素系统造成损害。在这项工作中,使用脂质体1,2-二硬脂酰基-sn-甘油基-3-磷酸乙醇胺-N-[甲氧基(聚乙二醇)-2000](DSPE-mPEG2000)包被的聚[(4,4'-双(2-乙基己基)-二噻吩并[3,2-b:2',3'-d]silole)-2,6-二基-alt-4,7-二(4-己基-2-噻吩基)-5,6-二氟-2,1,3-苯并噻二唑](PDTS-hDTBT)和聚[(4,4'-双(2-乙基己基)-二噻吩并[3,2-b:2',3'-d]silole)-2,6-二基-alt-4,7-二(4-(2-乙基己基)-2,3-噻吩基)-5,6-二氟-2,1,3-苯并噻二唑](PDTS-ehDTBT)。这两种聚合物具有不同的侧链,这会影响聚合物的结构,从而影响BPA和CPNP之间的π-π相互作用。探索了所得的两个CPNP作为酪氨酸酶固定化的极具吸引力的基质,以构建用于水环境中BPA的灵敏,快速检测的电化学生物传感平台。这两种生物传感器的电化学性能显著增强,得益于CPNPs的大比表面积和优异的生物相容性,以及CPNP和BPA之间强烈的π-π相互作用。PDTS-ehDTBT-Tyr-Chi/GCE的电流响应与BPA浓度在0.02至3.0μM之间表现出良好的线性关系,低检测限为11.83nM,高灵敏度为0.9724μAμM-1cm-2。制作的生物传感器进一步用于实际样品中的BPA检测,回收率为92.0%-99.4%。凭借显著的优势,基于CPNPs的生物传感器为实际样品中BPA的快速检测提供了高灵敏度的检测工具,具有广阔的应用前景。
Bisphenol A (BPA) has been widely used in the production of polycarbonate (PC) plastics, flame retardants and epoxy resins, which is one of the most important endocrine disrupting chemicals and can cause damage to the estrogen system of human. In this work, organic conjugated polymer nanoparticles (CPNPs) were synthesized through nanoprecipitation method using liposome 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[methoxy (polyethylene glycol)-2000] (DSPE-mPEG2000) coated poly[(4,4\'-bis(2-ethylhexyl)-dithieno[3,2-b:2\',3\'-d]silole)-2,6-diyl-alt-4,7-di(4-hexyl-2-thienyl)-5,6-difluoro-2,1,3-benzothiadiazole] (PDTS-hDTBT) and poly[(4,4\'-bis(2-ethylhexyl)-dithieno[3,2-b:2\',3\'-d]silole)-2,6-diyl-alt-4,7-di(4-(2-ethylhexyl)-2-thienyl)-5,6-difluoro-2,1,3-benzothiadiazole] (PDTS-ehDTBT). These two polymers have different side chains, which can affect the configuration of the polymers, thereby affecting the π-π interaction between BPA and CPNPs. The resultant two CPNPs were explored as extremely attractive matrix for tyrosinase immobilization to construct electrochemical biosensing platforms for sensitive and rapid detection of BPA in water environments. The electrochemical performance of these two biosensors was significantly enhanced, benefiting from the large specific surface area and excellent biocompatibility of CPNPs, as well as the strong π-π interaction between CPNPs and BPA. The current response of PDTS-ehDTBT-Tyr-Chi/GCE exhibited a good linear relationship with BPA concentration ranging from 0.02 to 3.0 μM with a low detection limit of 11.83 nM and a high sensitivity of 0.9724 μA μM-1 cm-2. The fabricated biosensor was further used for BPA detection in actual samples with a recovery rate of 92.0 %-99.4 %. With the remarkable advantages, CPNPs-based biosensor provides a highly sensitive detection tool for rapid detection of BPA in actual samples, which has broad application prospects.