在这项研究中,通过固定结肠癌和邻近组织(肿瘤两侧的周围健康组织)开发了一种电化学传感器,用于研究葡萄糖的受体传感动力学,味精,肌苷酸二钠,和乳酸钠.结果表明,由配体-受体相互作用触发的电信号呈现双曲线动力学特征,类似于酶与其底物的相互作用。结果表明,葡萄糖和谷氨酸钠的结肠癌组织和邻近组织的活化常数值相差两个数量级,肌钙二钠的活化常数值相差约一个数量级。癌组织没有感觉到乳酸钠,而邻近的组织可以感觉到乳酸钠。与正常细胞相比,癌细胞的营养感知能力显著提高,癌细胞感知能力的提高主要依赖于细胞内信号的级联放大。然而,与肿瘤邻近组织不同,结肠癌细胞失去感觉乳酸的能力。这为癌细胞的Warburg效应提供了关键证据。本研究的方法和结果有望为癌症研究提供新的思路,治疗,抗癌药物的筛选,和临床诊断。
In this
study, an electrochemical sensor was developed by immobilizing colon cancer and the adjacent tissues (peripheral healthy tissues on both sides of the tumor) and was used to investigate the receptor sensing kinetics of glucose, sodium glutamate, disodium inosinate, and sodium lactate. The results showed that the electrical signal triggered by the ligand-receptor interaction presented hyperbolic kinetic characteristics similar to the interaction of an enzyme with its substrate. The results indicated that the activation constant values of the colon cancer tissue and adjacent tissues differed by two orders of magnitude for glucose and sodium glutamate and around one order of magnitude for disodium inosinate. The cancer tissues did not sense sodium lactate, whereas the adjacent tissues could sense sodium lactate. Compared with normal cells, cancer cells have significantly improved nutritional sensing ability, and the improvement of cancer cells\' sensing ability mainly depends on the cascade amplification of intracellular signals. However, unlike tumor-adjacent tissues, colon cancer cells lose the ability to sense lactate. This provides key evidence for the Warburg effect of cancer cells. The methods and results in this
study are expected to provide a new way for cancer research, treatment, the screening of anticancer drugs, and clinical diagnoses.