Abstract：
Estrogen receptor alpha (ERα) serves as a crucial biomarker for early breast cancer diagnosis. In this study, we proposed an electrochemical aptasensor with nanomaterial carbon nanohorns/gold nanoparticle composites (1-AP-CNHs/AuNPs) as the substrate, and the primary amine groups on the antibody initiated the ring-opening polymerization (ROP) of monomer amino acid-ferrocene (NCA-Fc) on the electrode surface for ultrasensitive detection of ERα. The composite of 1-AP-CNHs/AuNPs not only possessed more active sites, but also increased the specific surface area of the electrode and allowed a large amount of ferrocene polymer long chains to be grafted onto the electrode surface to achieve signal amplification. Under optimal conditions, the detection limit of the method was 11.995 fg mL-1 with a detection range of 100 fg mL-1-100 ng mL-1. In addition, the biotin-streptavidin system was used to further improve the sensitivity of the sensor. Importantly, this approach could be applied for the practical detection of ERα in real samples.
摘要：
雌激素受体α（ERα）是早期乳腺癌诊断的重要生物标志物。在这项研究中,我们提出了一种以纳米材料碳纳米角/金纳米颗粒复合材料（1-AP-CNHs/AuNPs）为基底的电化学传感器，和抗体上的伯胺基团引发了电极表面单体氨基酸-二茂铁（NCA-Fc）的开环聚合（ROP），用于超灵敏检测ERα。1-AP-CNHs/AuNPs复合物不仅具有更多的活性位点,而且还增加了电极的比表面积，并使大量的二茂铁聚合物长链接枝到电极表面以实现信号放大。在最优条件下,该方法的检出限为11.995fgmL-1，检测范围为100fgmL-1-100ngmL-1。此外,生物素-链霉亲和素系统用于进一步提高传感器的灵敏度。重要的是,该方法可用于实际样品中ERα的实际检测。
