背景:猫是许多血液病原体的宿主和储库,例如,立克次体,嗜血支原体,巴尔通体,埃里希亚,和无性体,它们是由各种媒介节肢动物传播的,其中一些具有人畜共患的关注。尽管值得注意的是,近年来Türkiye伴侣动物的拥有率有所增加,而猫在这些动物中占很大比例,对它们携带的媒介传染病原体的研究有限。本研究旨在提供猫媒介传播的血液病原体(FVBHs)的全面分子流行病学数据和分子特征,包括牙质,无性体科,Rickettsias,血液病,和蒂尔基耶的巴尔通体物种。总的来说,从客户拥有的猫(n=203)和庇护所猫(n=47)中收集了250个猫科动物血样,这些猫被带到了Selcuk大学的小动物医院,兽医学院。
结果:总体而言,发现40只(16%)猫感染了至少一种被调查的血液病原体和piropasma,支原体属。和巴尔通菌属。患病率为1.6%,11.2%,和4.8%,分别。无无菌血症/埃里希菌属。和立克次体。在所研究的猫科动物样品中检测到DNA。序列分析显示,所有四个梨质都属于卵贝贝虫,与西班牙和Türkiye的18SrRNA基因序列具有97.93-99.82%的核苷酸序列同一性,虽然一些测序的血支原体是血支原体(Mhf),念珠菌支原体痰液(CMhm)和温尼氏支原体,和巴尔通菌属。是巴尔通氏菌和巴尔通氏菌。与支原体属共感染。和巴尔通菌属。在这项研究中,4只猫(1.6%)也被检测到,单一感染占主导地位。
结论:这项研究提供了关于Türkiye重要的猫科动物媒介传播的血液病原体的有价值的信息,其中一些在单一健康的观点下受到了关注,并且是第一个分子流行病学研究证明卵巴贝虫的存在,羊巴贝斯虫病的病原体,和温尼氏支原体DNA,牛造血支原体病的病原体,在猫。对在非特异性宿主中检测到的此类病原体的作用以及传播它们的载体的宿主特异性的进一步研究将有助于阐明这种情况。
BACKGROUND: Cats are hosts and reservoirs for many haemopathogens such as piroplasms, Rickettsia, hemotropic Mycoplasma, Bartonella, Ehrlichia, and Anaplasma, which are transmitted by various vector arthropods and some of which have a zoonotic concern. Although it is noteworthy that the rate of ownership of companion animals has increased in Türkiye in recent years and that cats account for a large proportion of these animals, there is limited research on the vector-borne infectious agents carried by them. The present study aimed to provide a comprehensive molecular epidemiological data and molecular characterization of feline vector-borne haemopathogens (FVBHs), including piroplasms, anaplasmataceae, rickettsias, haemoplasmas, and Bartonella species in Türkiye. In total, 250 feline blood samples were collected from client-owned cats (n = 203) and shelter cats (n = 47) brought to the Small Animal Hospital of Selcuk University, Veterinary Faculty.
RESULTS: Overall, 40 (16%) cats were found to be infected with at least one of the investigated haemopathogens and piroplasm, Mycoplasma spp. and Bartonella spp. prevalence was 1.6%, 11.2%, and 4.8%, respectively. No Anaplasma/Ehrlichia spp. and Rickettsia spp. DNA was detected in the investigated feline samples. Sequence analysis revealed that all four piroplasms belonged to Babesia ovis with a 97.93-99.82% nucleotide sequence identity to 18S rRNA gene sequences from Spain and Türkiye, while some sequenced hemoplasmas were Mycoplasma haemofelis (Mhf), Candidatus Mycoplasma haemominutum (CMhm) and Mycoplasma wenyonii, and Bartonella spp. were Bartonella henselae and Bartonella koehlerae species. Co-infections with Mycoplasma spp. and Bartonella spp. were also detected in 4 cats (1.6%) in this study, where single infections were predominant.
CONCLUSIONS: This study provides valuable information on zoonotically important feline vector-borne hemopathogens in Türkiye, some of which have received attention under the One Health perspective, and is the first molecular epidemiological study to demonstrate the presence of Babesia ovis, the causative agent of ovine
babesiosis, and Mycoplasma wenyonii DNA, the causative agent of bovine haemotropic mycoplasmosis, in cats. Further studies on the roles of such pathogens detected in unspecific hosts and the host specificity of the vectors that transmit them will contribute to the elucidation of this situation.