阿特拉津(ATRA)和环丙沙星(CPRO)被广泛检测,持久性和共存的水生污染物。这项研究调查了14天单次和联合ATRA和CPRO暴露对幼年Clariasgariepinus的影响。标准的生物测定方法被用来确定氧化应激的反应,肝脏状况,和免疫生物标志物在第7天和第14天。使用七组:对照组,CPROEC,CPROSubl,ATRAEC,ATRASubl,CPROEC+ATRAEC,和CPROSubl+ATRASubl。受试物质导致超氧化物歧化酶活性降低,过氧化氢酶,和谷胱甘肽过氧化物酶.脂质过氧化升高,特别是在CPRO-ATRA混合物中。血清转氨酶(ALT,和AST),碱性磷酸酶活性显著增加。总蛋白质,白蛋白,总免疫球蛋白,呼吸爆发明显减少。因此,单一和联合暴露于CPRO和ATRA会对水生生物造成不利影响。
Atrazine (ATRA) and ciprofloxacin (CPRO) are widely detected, persistent and co-existing aquatic pollutants. This study investigated effects of 14-day single and joint ATRA and CPRO exposure on juvenile Clarias gariepinus. Standard bioassay methods were used to determine responses of oxidative stress, hepatic condition, and immunological biomarkers on days 7 and 14. Seven groups were used: Control, CPROEC, CPROSubl, ATRAEC, ATRASubl, CPROEC+ATRAEC, and CPROSubl+ATRASubl. The test substances caused decreased activity of superoxide dismutase, catalase, and glutathione peroxidase. Lipid peroxidation was elevated, especially in CPRO-ATRA mixtures. Serum aminotransferases (ALT, and AST), and alkaline phosphatase activity increased significantly. Total protein, albumin, total immunoglobulin, and respiratory burst decreased significantly. Therefore, single and joint exposure to CPRO and ATRA poses adverse consequences on aquatic life.