PDF(Pubmed)
Abstract：
Annual variations in animal\'s physiological functions are an essential strategy to deal with seasonal challenges which also vary according to the time of year. Information regarding annual adaptations in the immune-competence to cope with seasonal stressors in reptiles is scarce. The present research plan was designed to analyze the presence of circannual immune rhythms in defense responses of the leucocytes in an ophidian, Natrix piscator. Peripheral blood leucocytes were obtained, counted, and superoxide anion production, neutrophil phagocytosis, and nitrite release were tested to assess the innate immune functions. Peripheral blood lymphocytes were separated by centrifugation (utilizing density gradient) and the cell proliferation was measured. The Cosinor rhythmometry disclosed the presence of significant annual rhythms in the number of leucocytes, superoxide anion production, nitric oxide production, and proliferation of stimulated lymphocytes. The authors found that respiratory burst activity and proliferative responses of lymphocytes were crucial immune responses that showed the annual rhythm. It was summarized that the immune function of the N. piscator is a labile attribute that makes the animal competent to cope with the seasonal stressor by adjustment in the potency of response.
摘要：
动物生理功能的年度变化是应对季节性挑战的基本策略，季节性挑战也因一年中的时间而异。有关爬行动物免疫能力年度适应以应对季节性压力的信息很少。本研究计划的目的是分析夜蛾白细胞的防御反应中每年一次的免疫节律的存在，NatrixPiscator.获得外周血白细胞，计数,和超氧阴离子的产生，中性粒细胞吞噬，测试和亚硝酸盐释放以评估先天免疫功能。通过离心(利用密度梯度)分离外周血淋巴细胞并测量细胞增殖。Cosinor节律测定法揭示了白细胞数量存在显著的年度节律，超氧阴离子生产，一氧化氮生产，和刺激的淋巴细胞的增殖。作者发现，淋巴细胞的呼吸爆发活动和增殖反应是显示年度节律的关键免疫反应。概述了N.piscator的免疫功能是一种不稳定的属性，它使动物有能力通过调节反应的效力来应对季节性应激源。
