Abstract：
BACKGROUND: Post-tuberculosis lung abnormality (PTLA) is the most common risk factor for chronic pulmonary aspergillosis (CPA), and 14%-25% of the subjects with PTLA develop CPA. The pathogenesis and the host immune response in subjects with PTLA who develop CPA need to be better understood.
METHODS: We prospectively compared the innate and adaptive immune responses mounted by patients of PTLA with or without CPA (controls). We studied the neutrophil oxidative burst (by dihydrorhodamine 123 test), classic (serum C3 and C4 levels) and alternative (mannose-binding lectin [MBL] protein levels) complement pathway, serum immunoglobulins (IgG, IgM and IgA), B and T lymphocytes and their subsets in subjects with PTLA with or without CPA.
RESULTS: We included 111 subjects (58 CPA and 53 controls) in the current study. The mean ± SD age of the study population was 42.6 ± 15.7 years. The cases and controls were matched for age, gender distribution and body weight. Subjects with CPA had impaired neutrophil oxidative burst, lower memory T lymphocytes and impaired Th-1 immune response (lower Th-1 lymphocytes) than controls. We found no significant difference between the two groups in the serum complement levels, MBL levels, B-cell subsets and other T lymphocyte subsets.
CONCLUSIONS: Subjects with CPA secondary to PTLA have impaired neutrophil oxidative burst and a lower Th-1 response than controls.
METHODS: We prospectively compared the innate and adaptive immune responses mounted by patients of PTLA with or without CPA (controls). We studied the neutrophil oxidative burst (by dihydrorhodamine 123 test), classic (serum C3 and C4 levels) and alternative (mannose-binding lectin [MBL] protein levels) complement pathway, serum immunoglobulins (IgG, IgM and IgA), B and T lymphocytes and their subsets in subjects with PTLA with or without CPA.
RESULTS: We included 111 subjects (58 CPA and 53 controls) in the current study. The mean ± SD age of the study population was 42.6 ± 15.7 years. The cases and controls were matched for age, gender distribution and body weight. Subjects with CPA had impaired neutrophil oxidative burst, lower memory T lymphocytes and impaired Th-1 immune response (lower Th-1 lymphocytes) than controls. We found no significant difference between the two groups in the serum complement levels, MBL levels, B-cell subsets and other T lymphocyte subsets.
CONCLUSIONS: Subjects with CPA secondary to PTLA have impaired neutrophil oxidative burst and a lower Th-1 response than controls.
摘要：
背景：结核后肺异常（PTLA）是慢性肺曲霉病（CPA）的最常见危险因素，14％-25％的PTLA受试者发展为CPA。需要更好地了解发展CPA的PTLA受试者的发病机理和宿主免疫反应。
方法：我们前瞻性比较了有或没有CPA（对照）的PTLA患者的先天和适应性免疫反应。我们研究了中性粒细胞氧化爆发（通过二氢罗丹明123测试），经典（血清C3和C4水平）和替代（甘露糖结合凝集素[MBL]蛋白水平）补体途径，血清免疫球蛋白（IgG，IgM和IgA），有或没有CPA的PTLA受试者的B和T淋巴细胞及其亚群。
结果：我们在本研究中纳入了111名受试者（58名CPA和53名对照）。研究人群的平均±SD年龄为42.6±15.7岁。病例和对照组的年龄相匹配，性别分布和体重。CPA受试者中性粒细胞氧化爆发受损，与对照组相比，较低的记忆T淋巴细胞和受损的Th-1免疫应答（较低的Th-1淋巴细胞）。我们发现两组之间的血清补体水平没有显着差异，MBL水平,B细胞亚群和其他T淋巴细胞亚群。
结论：PTLA继发CPA的受试者中性粒细胞氧化爆发受损，Th-1反应低于对照组。
方法：我们前瞻性比较了有或没有CPA（对照）的PTLA患者的先天和适应性免疫反应。我们研究了中性粒细胞氧化爆发（通过二氢罗丹明123测试），经典（血清C3和C4水平）和替代（甘露糖结合凝集素[MBL]蛋白水平）补体途径，血清免疫球蛋白（IgG，IgM和IgA），有或没有CPA的PTLA受试者的B和T淋巴细胞及其亚群。
结果：我们在本研究中纳入了111名受试者（58名CPA和53名对照）。研究人群的平均±SD年龄为42.6±15.7岁。病例和对照组的年龄相匹配，性别分布和体重。CPA受试者中性粒细胞氧化爆发受损，与对照组相比，较低的记忆T淋巴细胞和受损的Th-1免疫应答（较低的Th-1淋巴细胞）。我们发现两组之间的血清补体水平没有显着差异，MBL水平,B细胞亚群和其他T淋巴细胞亚群。
结论：PTLA继发CPA的受试者中性粒细胞氧化爆发受损，Th-1反应低于对照组。
