构建了米糠蛋白原纤维(RBPF)-高内相Pickering乳液(HIPPEs),负载了β-胡萝卜素(CE),以增强CE的稳定性和生物利用度。用不同贮藏期(0-10天)从不同氧化程度的米糠(RB)中提取不同氧化程度的RB蛋白,通过酸加热(90℃,2-12h)以稳定HIPPE。研究了蛋白质氧化对RBPF-HIPPEs包封性能的影响。结果表明,CE-HIPPE可以在25℃下稳定储存56天。当RB存储时间相同时,平均粒径,脂质过氧化氢含量,CE-HIPPEs的丙二醛含量和CE降解率初步下降,然后随着酸加热时间的延长而增长,而ζ电位值,粘度,粘弹性,游离脂肪酸(FFA)释放速率,和生物可及性第一玫瑰,随后倒下。当RBPF的酸加热时间相同时,平均粒径,脂质过氧化氢含量,CE-HIPPE的丙二醛含量最初下降,随后随着RB存储时间的延长而增加,而ζ电位值,粘度,粘弹性,FFA释放速率,生物可及性最初增加,然后下降。总的来说,适度的氧化和适度的酸加热增强了CE-HIPPE的稳定性和流变性能,从而提高CE的稳定性和生物可及性。这项研究为基于蛋白质原纤维聚集体的HIPPE递送生物活性物质提供了新的见解。
Rice bran protein fibril (RBPF)-high internal phase Pickering emulsions (HIPPEs) loaded with β-carotene (CE) were constructed to enhance stability and bioavailability of CE. Rice bran (RB) protein with varying oxidation degrees was extracted from RB with varying storage period (0-10 days) to prepare RBPF by acid-heating (90 °C, 2-12 h) to stabilize HIPPEs. The influence of protein oxidation on the encapsulation properties of RBPF-HIPPEs was studied. The results showed that CE-HIPPEs could be stably stored for 56 days at 25 °C. When RB storage time was the same, the average particle size, lipid hydroperoxide content, and malondialdehyde content of CE-HIPPEs and the CE degradation rate initially fell, and then grew as the acid-heating time prolonged, while the ζ-potential value, viscosity, viscoelasticity, free fatty acid (FFA) release rate, and bioaccessibility first rose, and subsequently fell. When acid-heating time of RBPF was the same, the average particle size, lipid hydroperoxide content, and malondialdehyde content of CE-HIPPEs initially fell, and subsequently increased with RB storage time extended, while the ζ-potential value, viscosity, viscoelasticity, FFA release rate, and bioaccessibility initially increased, and then decreased. Overall, Moderate oxidation and moderate acid-heating enhanced the stability as well as rheological properties of CE-HIPPEs, thus improving the stability and bioaccessibility of CE. This study offered a new insight into the delivery of bioactive substances by protein fibril aggregates-based HIPPEs.