Abstract：
In this study, high internal phase Pickering emulsions (HIPPEs) were stabilized by the complexes of peanut protein isolate (PPI) and cellulose nanocrystals (CNCs) for encapsulation β-carotene to retard its degradation during processing and storage. CNCs were prepared by H2SO4 hydrolysis (HCNCs), APS oxidation (ACNCs) and TEMPO oxidation (TCNCs), exhibiting needle-like or rod-like structures with nanoscale size and uniformly distributed around the spherical PPI particle, which enhanced the emulsifying capability of PPI. Results of optical micrographs and droplet size measurement showed that Pickering emulsions stabilized by PPI/ACNCs complexes exhibited the most excellent stability after 30 days of storage, which indicated that ACNCs had the most obvious effect to improve emulsifying capability of PPI. HIPPEs encapsulated β-carotene (βc-HIPPEs) were stabilized by PPI/ACNCs complexes and showed excellent inverted storage stability. Moreover, βc-HIPPEs exhibited typical shear thinning behavior investigated by rheological properties analysis. During thermal treatment, ultraviolet radiation and oxidation, the retentions of β-carotene encapsulated in HIPPEs were improved significantly. This research holds promise in expanding Pickering emulsions stabilized by proteins-polysaccharide particles to delivery systems for hydrophobic bioactive compounds.
摘要：
在这项研究中,高内相Pickering乳液（HIPPEs）通过花生分离蛋白（PPI）和纤维素纳米晶体（CNCs）的复合物稳定，用于包封β-胡萝卜素，以延缓其在加工和储存过程中的降解。CNCs通过H2SO4水解(HCNCs)制备,APS氧化（ACNC）和TEMPO氧化（TCNCs），表现出针状或棒状结构，具有纳米级尺寸，均匀分布在球形PPI颗粒周围，提高了PPI的乳化能力。光学显微照片和液滴尺寸测量结果表明，由PPI/ACNCs复合物稳定的Pickering乳液在储存30天后表现出最优异的稳定性，表明ACNCs对PPI乳化能力的改善作用最为明显。HIPPE封装的β-胡萝卜素（βc-HIPPE）通过PPI/ACNC复合物稳定，并显示出优异的反转储存稳定性。此外，βc-HIPPE表现出典型的剪切稀化行为，通过流变特性分析进行了研究。在热处理期间，紫外线辐射和氧化，包封在HIPPE中的β-胡萝卜素的保留率显着提高。这项研究有望将由蛋白质-多糖颗粒稳定的Pickering乳液扩展到疏水性生物活性化合物的递送系统。
