Abstract：
Recently, there has been a focus on using biopolymer-based particles to stabilize high internal phase Pickering emulsions (HIPPEs) due to the notable advances in biocompatibility and biodegradability. In this work, the complex particles of peanut protein isolate and carboxymethyl cellulose (CMC) with various substitution degrees (DS; 0.7 and 0.9) and weight average molecular weights (Mw; 90, 250, and 700 kDa) were prepared and characterized as novel stabilizers. For the obtained four types of morphologically distinct particles, the complex particles formed by CMC (0.9 DS and 250 kDa) showed cluster structures with an average size of 1.271 μm, equally biphasic wettability with three-phase contact angles of 91.5°, and the highest diffusion rate at the oil-water interface. HIPPEs stabilized by these particles exhibited more elastic behavior due to the smaller tanδ and higher viscosity, as well as excellent thixotropic recovery properties and stability against heating, storage, and freeze-thawing. Furthermore, confocal laser scanning microscopy verified that these particles formed a dense interfacial layer around the oil droplets, which could resist flocculation and coalescence between oil droplets during in vitro digestion. The improved bioaccessibility of curcumin-loaded HIPPEs made these delivery systems potentially apply in functional foods.
摘要：
最近,由于在生物相容性和生物降解性方面的显著进步,一直关注使用基于生物聚合物的颗粒来稳定高内相Pickering乳液(HIPPE)。在这项工作中,制备了具有不同取代度（DS;0.7和0.9）和重均分子量（Mw;90、250和700kDa）的花生分离蛋白和羧甲基纤维素（CMC）的复合颗粒，并将其表征为新型稳定剂。对于获得的四种形态上不同的颗粒，由CMC（0.9DS和250kDa）形成的复合颗粒显示出平均尺寸为1.271μm的簇结构，三相接触角为91.5°的等效双相润湿性，并且在油水界面处的扩散速率最高。由这些颗粒稳定的HIPPE由于较小的tanδ和较高的粘度而表现出更多的弹性行为。以及优异的触变性恢复性能和抗热稳定性，storage,和冻融。此外，共聚焦激光扫描显微镜验证了这些颗粒在油滴周围形成了致密的界面层，在体外消化过程中可以抵抗油滴之间的絮凝和聚结。姜黄素负载的HIPPE的改善的生物可及性使得这些递送系统潜在地应用于功能性食品。
