氧化锌纳米颗粒(ZnONPs)是化妆品和外用产品中使用最丰富的纳米材料之一,如今,它们在药物递送和组织工程方面进行了探索。最近的一些数据证明它们是心脏毒性作用和全身毒性的原因。本研究旨在研究ZnONPs(39nm)对Wistar大鼠心脏的毒性作用,并使用三种不同剂量水平(25、50、100mg/kgbw)的ZnONPs进行剂量反应关系心电图(ECG)读数,心脏生化功能参数的水平,以及氧化应激和抗氧化生物标志物。此外,测定锌浓度水平和心脏组织病理学检查。ZnONPs表现出剂量依赖性效应,因为100mg/kgbwZnONPs治疗组表现出最显著的心电图参数变化:R-R距离,P-R间隔,R和T振幅,和心脏酶肌酸激酶-MB(CK-MB)和乳酸脱氢酶(LDH)的水平增加。另一方面,锌浓度升高,氧化应激生物标志物和MDA,GSH水平下降也呈剂量依赖性,心脏组织的组织病理学结构受损支持了这一结果.而100mg/kgbw剂量的ZnO散装组对心功能无明显影响。本研究得出结论,ZnONPs主要在高剂量时可引起心功能障碍和病理损害。
Zinc oxide nanoparticles (ZnO NPs) are one of the most abundantly used nanomaterials in cosmetics and topical products, and nowadays, they are explored in drug delivery and tissue engineering. Some recent data evidenced that they are responsible for cardiotoxic effects and systemic toxicity. The present study aimed to investigate the toxic effect of ZnO NPs (39 nm) on the
heart of Wistar rats and to perform a dose-response relationship using three different dose levels (25, 50, 100 mg/kg bw) of ZnO NPs on the electrocardiogram (ECG) readings, the levels of biochemical function parameters of
heart, and the oxidative stress and antioxidant biomarkers. Furthermore, zinc concentration level and histopathological examination of
heart tissues were determined. ZnO NPs showed a dose-dependent effect, as the 100 mg/kg bw ZnO NPs treated group showed the most significant changes in ECGs parameters: R-R distance, P-R interval, R and T amplitudes, and increased levels of
heart enzymes Creatine Kinase- MB (CK-MB) and Lactate dehydrogenase (LDH). On the other hand, elevated zinc concentration levels, oxidative stress biomarkers MDA and NO, and decreased GSH levels were found also in a dose-dependent manner, the results were supported by impairment in the histopathological structure of heart tissues. While the dose of 100 mg/kg bw of ZnO bulk group showed no significant effects on
heart function. The present study concluded that ZnO NPs could induce cardiac dysfunctions and pathological lesions mainly in the high dose.