背景:\'KarkatakaTaila(KT),一种古老的阿育吠陀Rasayana,包括可食用的淡水蟹ScyllaserrataForskal肉,喀拉拉邦的当地传统从业者仍在使用它来治疗震颤和麻痹。在科学界,由于缺乏足够的科学验证和简短的报告,它变得不那么暴露。目前尚无关于KT治疗帕金森病(PD)有效性的公开研究。
目的:目前的研究工作的目的是研究KT对SH-SY5Y细胞系和PD大鼠模型中鱼藤酮诱导的神经毒性的抗Parkison的潜力,并探讨潜在的分子机制。
方法:通过气相色谱-质谱(GC-MS)鉴定了KT的成分。使用SH-SY5Y细胞系和大鼠评估KT对鱼藤酮诱导的PD的神经保护活性。用于评估神经保护的参数是抗氧化剂标记(ROS和SOD),抗炎标志物(IL-6,IL-1β,TNF-α,和亚硝酸盐),和多巴胺水平。进行行为评估和大鼠脑组织病理学以进一步支持神经保护。
结果:使用GC-MS的分析显示KT中有36种成分。体外,KT在降低氧化应激(ROS和SOD)方面表现出相当大的神经保护作用,神经炎症(IL-6,IL-1β,TNF-α,和亚硝酸盐),并提高多巴胺浓度.体内数据显示组织病理学和生化参数的改善证实了体外研究结果,就行为分析而言,KT显示出显著的活性。
结论:GC-MS分析用于鉴定具有抗氧化剂的KT的生物活性化合物,抗炎,和神经保护特性。因此,它们可能与KT对PD的治疗作用有关。
BACKGROUND: \'Karkataka Taila (KT), an ancient Ayurvedic Rasayana comprising the edible freshwater crab Scylla serrata Forskal flesh, is still used by local traditional practitioners in Kerala state to treat tremors and palsy. In the scientific community, it becomes less exposed due to the lack of adequate scientific validations and brief reports. There has been no published research on the effectiveness of KT in treating Parkinson\'s disease (PD).
OBJECTIVE: The purpose of the current research work was to investigate the anti-Parkison\'s potential of KT against rotenone-induced neurotoxicity in SH-SY5Y cell lines and rat model of PD and investigate underlying molecular mechanisms.
METHODS: The components of KT have been identified by gas chromatography-mass spectroscopy (GC-MS). The neuroprotective activity of KT was assessed using SH-SY5Y cell lines and rats against rotenone-induced PD. The parameters used for asses the neuroprotection are antioxidant markers (ROS and SOD), anti-inflammatory markers (IL-6, IL-1β, TNF-α, and nitrite), and dopamine levels. Behavioral evaluation and rat brain histopathology were carried out to further support the neuroprotection.
RESULTS: Analysis using GC-MS revealed 36 constituents in KT. In vitro, the KT displayed considerable neuroprotective effects in terms of decreasing oxidative stress (ROS and SOD), neuroinflammation (IL-6, IL-1β, TNF-α, and nitrite), and elevating dopamine concentration. In vivo data showing improvements in histopathological and biochemical parameters confirmed the in vitro study findings, and in terms of behavioral assays, KT displayed significant activity.
CONCLUSIONS: GC-MS profiling was used to identify the bioactive compounds of KT with antioxidant, anti-inflammatory, and neuroprotective properties. As a result, they may be responsible for the therapeutic effects of KT on PD.