大豆异黄酮(SI)广泛存在于食品和草药中。虽然SIs的药理活性已被广泛报道,它们对正常宿主肠道微生态的影响很少受到关注。给予5周龄的昆明(KM)小鼠SIs(10mg/kg/天)15天。食物摄入量,体重,并测定消化酶活性。小肠微生物群,包括管腔相关细菌(LAB)和粘膜相关细菌(MAB),使用16S核糖体核糖核酸(16SrRNA)基因测序进行分析。短链脂肪酸(SCFA)使用气相色谱-质谱(GC-MS)分析。结果表明,食用SI的小鼠比正常小鼠的食物摄入量高,但体重增加率低。蔗糖,纤维素酶,淀粉酶活性减少,而SIs干预后蛋白酶活性增加。此外,SIs增加了正常小鼠的LAB和MAB中的肠道细菌多样性。LAB的组成比MAB对SI更敏感。乳酸菌,Adlercreutzia,球菌,Ruminococus,Butyricicocus,和脱硫弧菌是用SI处理的小鼠的LAB中的差异细菌。此外,乙酸,戊酸,异丁酸,异戊酸,己酸减少,而用SI处理的小鼠中丁酸和丙酸增加。一起来看,SI有利于控制体重,即使是短期干预。其具体机制与调节肠道菌群有关,改变消化酶的活性,并进一步影响碳水化合物的吸收和代谢。
Soybean isoflavones (SIs) are widely found in food and herbal medicines. Although the pharmacological activities of SIs have been widely reported, their effects on the intestinal microecology of normal hosts have received little attention. Five-week-old Kunming (KM) mice were administered SIs (10 mg/kg/day) for 15 days. Food intake, body weight, and digestive enzyme activity were measured. Small intestine microbiota, including lumen-associated bacteria (LAB) and mucosa-associated bacteria (MAB), were analyzed using 16S ribosomal ribonucleic acid (16S rRNA) gene sequencing. Short-chain fatty acids (SCFAs) were analyzed using gas chromatography-mass spectrometry (GC-MS). The results showed that the mice that consuming SIs showed a higher food intake but a lower body weight gain rate than that of normal mice.
Sucrase, cellulase, and amylase activities reduced, while protease activity increased after SIs intervention. Moreover, SIs increased the intestinal bacterial diversity in both LAB and MAB of normal mice. The composition of LAB was more sensitive to SIs than those of MAB. Lactobacillus, Adlercreutzia, Coprococcus, Ruminococcus, Butyricicoccus, and Desulfovibrio were the differential bacteria among the LAB of mice treated with SIs. In addition, acetic acid, valeric acid, isobutyric acid, isovaleric acid, and caproic acid decreased, while butyric acid and propionic acid increased in the mice treated with SIs. Taken together, SIs are beneficial for weight control, even in short-term interventions. The specific mechanism is related to regulating the gut microbiota, changing digestive enzyme activities, and further affecting carbohydrate absorption and metabolism.