使用冷却运输精液进行人工授精在世界各地的马育种计划中具有重要意义。骡子的高价值引起了人们对驴精液生物技术的狂热兴趣。然而,驴精液在市售的马补充剂中冷却不良。因此,本研究旨在研究提高驴精液耐冷能力的方法。在三种不同的补充剂(基于牛奶的,SM;酪蛋白酸钠基,SC;或基于蛋黄的,EY)在存在或不存在精浆的情况下(离心,C).精子运动,质膜完整性(PMI),质膜稳定性(PMS),线粒体膜电位(HMMP),细胞内过氧化氢(H2O2),和细胞内超氧化物(O2-)之前进行了评估,24h,和48小时后冷却。此外,在其中一种处理下(SM,SC,EY,SM-C,SC-C,或EY-C)。团体EY,SC-C,在24和48h时,EY-C(P<0.05)显示出优于SM的精子分析参数。离心对SM和SC中延伸的冷却驴精液的精子分析参数有积极影响(P<0.05)。在SC繁殖的母马精液延长(67%,18/27),SC-C(89%,24/27),安永(89%,25/28),或EY-C(74%,20/27)的受胎率明显高于SM繁殖的母马(33%,9/27;P<0.05)。用SM-C繁殖的母马的受孕率中等(59%,16/27).总之,SC和EY提高了马母马驴精液的冷却能力和繁殖力,离心对SM中延伸的驴精液有积极影响。
Artificial insemination using cooled-transported semen has marked importance in equine breeding programs around the world, and the high value of mules has generated avid interest in donkey semen biotechnology. However, donkey semen cools poorly in commercially available equine extenders. Therefore, this study aimed to develop approaches to improve the ability of donkey semen to tolerate cooling. Ejaculates of seven donkeys (n = 21) were cooled at 5°C for 48 h in three different extenders (milk-based, SM; sodium caseinate-based, SC; or egg yolk-based, EY) in the presence or absence of seminal plasma (centrifugation, C). Sperm motility, plasma membrane integrity (PMI), plasma membrane stability (PMS), mitochondrial membrane potential (HMMP), intracellular hydrogen peroxide (H2O2), and intracellular superoxide ( O 2 - ) were assessed before, 24 h, and 48 h post-cooling. In addition, 15 mares (163 estrous cycles) were randomly inseminated with semen from two jacks (Jack 1, n = 90; Jack 2, n = 73) previously cooled for 24 h under one of the treatments (SM, SC, EY, SM-C, SC-C, or EY-C). Groups EY, SC-C, and EY-C (P < 0.05) demonstrated superior sperm analytical parameters to SM at 24 and 48 h. Centrifugation positively affected sperm analytical parameters in cooled donkey semen extended in SM and SC (P < 0.05). Mares bred with semen extended in SC (67%, 18/27), SC-C (89%, 24/27), EY (89%, 25/28), or EY-C (74%, 20/27) had significantly greater conception rates than mares bred with SM (33%, 9/27; P < 0.05). Mares bred with SM-C had intermediate conception rates (59%, 16/27). In conclusion, SC and EY improved the cooling ability and fertility of donkey semen in horse mares, and centrifugation positively affected donkey semen extended in SM.