PDF(Pubmed)
Abstract：
Bee drone brood is a beehive by-product with high hormonal activity used in natural medicine to treat male infertility. The aim of the study was to assess the effect of drone brood on stallion spermatozoa during a short-term incubation for its potential use in the equine semen extenders. Three different forms of fixed drone brood (frozen (FR), freeze-dried (FD), and dried extract (DE)) were used. Solutions of drone brood were compared in terms of testosterone, protein, total phenolic content, and antioxidant activity. The stallion semen was diluted with prepared drone brood solutions. The computer-assisted semen analysis (CASA) method was employed to evaluate the movement characteristics of the diluted ejaculate. To determine spermatozoa viability, the mitochondrial toxicity test (MTT) and Alamar Blue test were performed. In terms of testosterone content and antioxidant activity, a close likeness between FR and FD was found whereas DE\'s composition differed notably. FR had a positive effect mainly on progressive motility, but also on sperm distance and speed parameters after 2 and 3 h of incubation. On the contrary, FD and DE acted negatively, depending on increasing dose and time. For the first time, a positive dose-dependent effect of fixed drone brood on spermatozoa survival in vitro was demonstrated.
摘要：
蜜蜂无人机育苗是一种具有高激素活性的蜂箱副产品，用于天然药物治疗男性不育症。该研究的目的是评估无人机在短期孵化过程中对种马精子的影响，以评估其在马精液补充剂中的潜在用途。三种不同形式的固定无人机育苗(冷冻(FR)，冻干（FD），使用干燥的提取物(DE))。无人机育苗的解决方案在睾丸激素方面进行了比较，蛋白质，总酚含量，和抗氧化活性。用制备的无人机育苗溶液稀释种马精液。采用计算机辅助精液分析（CASA）方法评估稀释射精的运动特征。为了确定精子的生存能力，进行线粒体毒性试验（MTT）和AlamarBlue试验。在睾酮含量和抗氧化活性方面，发现FR和FD相似，而DE的组成明显不同。FR主要对渐进性运动有积极影响，还要对精子孵化2和3小时后的距离和速度参数。相反,FD和DE采取负面行动，取决于剂量和时间的增加。第一次,证明了固定无人机对精子体外存活的正剂量依赖性作用。
