布鲁氏菌病是一种广泛分布于世界各地的传染病,在一些国家,它被认为是一个公共卫生问题。布鲁氏菌病引起隐匿的症状,使其难以诊断。感染也可引发慢性疼痛和神经精神并发症。抗生素并不总是有效地根除感染,有助于慢性。我们的目的是研究抗生素治疗对促炎细胞因子,神经递质,皮质酮,和在流产芽孢杆菌2308株感染小鼠模型中的行为。创建了四个研究组:(a)对照;(b)抗生素对照;(c)用流产芽孢杆菌2308感染;和(d)用利福平和强力霉素感染和治疗。我们确定了流产芽孢杆菌2308个菌落形成单位(CFU),树突状细胞的计数,和巨噬细胞在脾脏;细胞因子和皮质酮的血清水平;血清素的水平,多巴胺,肾上腺素,大脑中的去甲肾上腺素;和平衡,体力,焦虑,和绝望测试。将感染和治疗的小鼠组与对照和感染的小鼠进行比较,以评估治疗是否足以恢复神经免疫内分泌参数。我们的结果表明,尽管用利福平和多西环素治疗布鲁氏菌病,抗生素治疗的小鼠表现出流产芽孢杆菌2308CFU的持久性,巨噬细胞数量的增加,和较高的皮质酮循环水平。此外,IL-12,IL-6和TNF-α的水平仍然较高。我们发现,随着海马神经递质的变化,肌肉力量和平衡能力下降,小脑,和额叶皮层.我们的数据表明,抗生素给药后剩余的细菌负荷有利于炎症,神经化学,和行为改变,部分解释了慢性布鲁氏菌病患者所经历的广泛和矛盾的症状学。
Brucellosis is an infection widely distributed around the world, and in some countries it is considered a public health problem. Brucellosis causes insidious symptoms that make it difficult to diagnose. Infection can also trigger chronic pain and neuropsychiatric complications. Antibiotics are not always effective to eradicate infection, contributing to chronicity. We aimed to investigate the effects of antibiotic treatment on proinflammatory cytokines, neurotransmitters, corticosterone, and behavior in a murine model of infecrion of B. abortus strain 2308. Four study groups were created: (a) control; (b) antibiotic control; (c) infected with B. abortus 2308; and (d) infected and treated with
rifampicin and doxycycline. We determined B. abortus 2308 colony-forming units (CFUs), the count of dendritic cells, and macrophages in the spleen; serum levels of cytokines and corticosterone; levels of serotonin, dopamine, epinephrine, and norepinephrine in the brain; and equilibrium, physical strength, anxiety, and hopelessness tests. The infected and treated mice group was compared with the control and infected mice to assess whether treatment is sufficient to recover neuroimmunoendocrine parameters. Our results showed that despite the treatment of brucellosis with
rifampicin and doxycycline, antibiotic-treated mice showed a persistence of B. abortus 2308 CFUs, an increased count in macrophage number, and higher circulating levels of corticosterone. Furthermore, the levels of IL-12, IL-6, and TNF-α remained higher. We found a decrease in muscular strength and equilibrium concomitant to changes in neurotransmitters in the hippocampus, cerebellum, and frontal cortex. Our data suggest that the remaining bacterial load after antibiotic administration favors inflammatory, neurochemical, and behavioral alterations, partly explaining the widespread and paradoxical symptomatology experienced by patients with chronic brucellosis.