杜仲是一种传统的中草药,具有降低血压和增强免疫功能等药理作用。研究了饲粮中添加杜仲多糖(EUP)对松辽黑猪免疫功能和肉品质的影响。血液淋巴细胞计数和百分比,血清总蛋白和白蛋白的浓度增加,而尿素氮和甘油三酯下降。白细胞和淋巴细胞计数,血清IgA,IgE,IgG2和IFN-γ增加。平均每日体重增加,屠宰重量,瘦肉率和熟肉率提高,而pH24,饲料重量比,脂肪率,黄度(b#)和离心脱水率降低。背最长肌的转录组测序检测到32个差异表达基因(DEGs),其中26个上调,6个下调。四组中共有19个基因差异表达,其中18个被上调。DEG包括ADAMTS4,PER1,STAC,SERPINE1,FASN,THRSP,SP7和KRT80与蛋白质相互作用网络显示20个上调节点,三个下调节点和14个DEG。GO功能注释和富集剖析显示,有34个项目显著富集,包括转移酶活性,肌动蛋白结合,乙酰辅酶A,酰基辅酶A代谢,脂肪组织发育和酰基甘油稳态。KEGG通路分析显示AMPK和PPAR信号通路被富集。饲粮杜仲多糖增强松辽黑猪免疫功能,提高生长和胴体性能,增加了与肉质性状相关基因的表达,改善了肉质。
Eucommia ulmoides is a traditional Chinese herbal medicine, with pharmacological effects such as lowering blood pressure and enhancing immune function. The effects of dietary Eucommia ulmoides polysaccharide (EUP) on immune function and meat quality were studied in Songliao Black Pigs. Blood lymphocyte counts and percentage, concentrations of serum total protein and of albumin increased, whereas those of urea nitrogen and triglyceride decreased. White blood cell and lymphocyte counts, and serum IgA, IgE, IgG2 a and IFN-γ increased. Average daily weight gain, slaughter weight, lean meat rate and cooked meat rate increased, whereas pH24, feed-weight ratio, fat rate, yellowness (b#) and centrifugal dehydration rate decreased. Transcriptome sequencing of longissimus dorsi muscle detected 32 differentially expressed genes (DEGs), of which 26 were up-regulated and 6 down-regulated. A total of 19 genes were differentially expressed in the four groups, 18 of which were up-regulated. The DEGs included ADAMTS4, PER1, STAC, SERPINE1, FASN, THRSP, SP7 and KRT80 and the protein interaction network showed 20 up-regulated nodes, three down-regulated nodes and 14 DEGs. GO functional annotation and enrichment analysis showed that 34 items were significantly enriched, including transferase activity, actin binding, acetyl coenzyme A, acyl coenzyme A metabolism, adipose tissue development and acyl glycerol homeostasis. KEGG pathway analysis showed that the AMPK and PPAR signaling pathways were enriched. Dietary Eucommia polysaccharide enhanced immune function in Songliao Black Pigs, improved growth and carcass performance, increased the expression of genes related to meat quality traits and improved meat quality.