辣椒是辣椒属(茄科)的成员。它们原产于中美洲和南美洲,由大约35种组成[1,2]。其中,五种(C.annuumL.,C.baccatumL.,C.中国的雅克。,C.frutescensL.,和C.pubescensRuiz&Pav。)已被驯化,主要种植用作蔬菜和香料。在驯化的辣椒中,C.annuum在全球范围内进行商业种植,C.frutescens和C.chinense主要在美国种植,亚洲人,非洲国家[3]。我们比较了农场栽培(FC)和家庭种植(HP)辣椒植物(辣椒)的各个隔室中微生物群的多样性。使用IlluminaNovaSeq6000平台对靶向的16SrRNA基因(V5-V6区)进行测序。变形杆菌,放线菌,酸杆菌,Gemmatimonadota,拟杆菌,FC和HP工厂的所有隔室中都存在Firmicutes。Proteobacteria(或Pseudomonadota)是HP和FC植物所有隔室中的主要门。而放线菌群(或放线菌群)是第二丰富的门。大多数植物隔室(叶子,果实和根)与土壤样品相比,变形杆菌的相对丰度更高。除了少数例外,土壤隔室(散装和根际土壤)显示出较高的门粘菌相对丰度,酸杆菌,Gemmatimonadota,拟杆菌,Nitrosirota,Verrucomicrobiota,和Firmicutes比植物隔间。多样性指数显示,辣椒植物中的细菌群落基于区室和栽培面积而聚集。
Chillies are members of the genus Capsicum L. (family Solanaceae). They are native to Central and South America and consist of approximately 35 species [1,2]. Among these, five species (C. annuum L., C. baccatum L., C. chinense Jacq., C. frutescens L., and C. pubescens Ruiz & Pav.) have been domesticated and are mainly cultivated for consumption as vegetables and spices. Of the domesticated chillies, C. annuum is commercially cultivated worldwide, while C. frutescens and C. chinense are mainly cultivated in American, Asian, and African countries [3]. We compared the diversity of microbiota in various compartments of farm-cultivated (FC) and home-planted (HP) chilli plants (Capsicum frutescens). Targeted 16S rRNA gene (V5-V6 region) was sequenced using the Illumina NovaSeq 6000 platform. Proteobacteria, Actinobacteriota, Acidobacteriota, Gemmatimonadota, Bacteroidota, and Firmicutes were present in all compartments of both the FC and HP plants. Proteobacteria (or Pseudomonadota) was the predominant phylum in all the compartments of both HP and FC plants, while Actinobacteriota (or Actinomycetota) was the second most abundant phylum. Most plant compartments (leaves, fruits and roots) exhibited a higher relative abundance of Proteobacteria compared to the soil samples. With few exceptions, the soil compartments (bulk and rhizospheric soils) displayed a higher relative abundance of the phyla Myxococcota, Acidobacteriota, Gemmatimonadota, Bacteroidota, Nitrospirota, Verrucomicrobiota, and Firmicutes than the plant compartments. Diversity indices revealed that the bacterial community in chili plants clustered based on both compartment and cultivation area.