微RNA(miRNA,miRs)和相关网络可能在不同利什曼原虫物种的差异宿主细胞感染过程中发挥关键作用。因此,开展了微阵列数据集的生物信息学分析,以鉴定利什曼病的关键共有生物标志物和基于miRNA的调控网络.通过使用一组全面的基因表达谱分析微阵列进行转录组学分析,以鉴定与利什曼原虫相关的关键基因和miRNA。感染。因此,将健康人对照的基因表达谱与感染墨西哥利什曼原虫的个体进行比较,L.少校,L.Donovani,还有L.Braziliensis.利用EnrichR数据库对数据集进行富集分析,和蛋白质-蛋白质相互作用(PPI)网络来识别集线器基因。通过使用受试者工作特征(ROC)曲线评估hub基因的预后价值。最后,使用miRTarBase鉴定与hub基因相互作用的miRNA,miRWalk,TargetScan,和mirnet。在本研究中比较的各组之间鉴定了差异表达的基因。这些基因在炎症反应中显著富集,细胞因子介导的信号通路和粒细胞和中性粒细胞趋化反应。对招募数据集的hub基因的鉴定表明,TNF,SOCS3,JUN,TNFAIP3和CXCL9可能是潜在的感染生物标志物,并且值得作为利什曼病的预后生物标志物。此外,来自miRWalk的推断数据揭示了许多miRNA的显着程度的相互作用(hsa-miR-8085,hsa-miR-4673,hsa-miR-4743-3p,hsa-miR-892c-3p,hsa-miR-4644,hsa-miR-671-5p,hsa-miR-7106-5p,hsa-miR-4267,hsa-miR-5196-5p,和hsa-miR-4252)与大多数hub基因,这表明这种miRNAs在寄生虫感染后起着至关重要的作用。在这项研究中鉴定的hub基因和hubmiRNAs可能被认为是治疗利什曼病的治疗靶标或生物标志物。
Micro RNAs (miRNAs, miRs) and relevant networks might exert crucial functions during differential host cell infection by the different Leishmania species. Thus, a bioinformatic analysis of
microarray datasets was developed to identify pivotal shared biomarkers and miRNA-based regulatory networks for Leishmaniasis. A transcriptomic analysis by employing a comprehensive set of gene expression profiling microarrays was conducted to identify the key genes and miRNAs relevant for Leishmania spp. infections. Accordingly, the gene expression profiles of healthy human controls were compared with those of individuals infected with Leishmania mexicana, L. major, L. donovani, and L. braziliensis. The enrichment analysis for datasets was conducted by utilizing EnrichR database, and Protein-Protein Interaction (PPI) network to identify the hub genes. The prognostic value of hub genes was assessed by using receiver operating characteristic (ROC) curves. Finally, the miRNAs that interact with the hub genes were identified using miRTarBase, miRWalk, TargetScan, and miRNet. Differentially expressed genes were identified between the groups compared in this study. These genes were significantly enriched in inflammatory responses, cytokine-mediated signaling pathways and granulocyte and neutrophil chemotaxis responses. The identification of hub genes of recruited datasets suggested that TNF, SOCS3, JUN, TNFAIP3, and CXCL9 may serve as potential infection biomarkers and could deserve value as prognostic biomarkers for leishmaniasis. Additionally, inferred data from miRWalk revealed a significant degree of interaction of a number of miRNAs (hsa-miR-8085, hsa-miR-4673, hsa-miR-4743-3p, hsa-miR-892c-3p, hsa-miR-4644, hsa-miR-671-5p, hsa-miR-7106-5p, hsa-miR-4267, hsa-miR-5196-5p, and hsa-miR-4252) with the majority of the hub genes, suggesting such miRNAs play a crucial role afterwards parasite infection. The hub genes and hub miRNAs identified in this study could be potentially suggested as therapeutic targets or biomarkers for the management of leishmaniasis.