类器官肿瘤模型已成为生物学和医学领域的强大工具,因为从肿瘤细胞生长的3D结构可以概括出更好的肿瘤特征。使这些肿瘤独特的个性化癌症研究。评估他们的功能行为,特别是蛋白质分泌,提供全面的见解非常重要。这里,提供了一种无标记的光谱成像平台,该平台具有先进的集成光流控纳米等离子体生物传感器,可对单个肿瘤进行实时分泌分析。一种新颖的双层微孔设计可分离肿瘤,防止信号干扰,和微阵列配置允许同时分析多个肿瘤。双成像功能结合了延时等离子体光谱和明场显微镜有助于同时观察分泌动力学,运动性,和形态学。整合的生物传感器已通过源自细胞系和患者样品的结直肠肿瘤来证明,以研究其血管内皮生长因子A(VEGF-A)的分泌。增长,和各种条件下的运动,包括常氧,缺氧,和药物治疗。这个平台,通过提供纳米光子学的无标签方法来监测肿瘤,可以为基础生物学研究的新应用铺平道路,药物筛选,以及疗法的发展。
Organoid tumor models have emerged as a powerful tool in the fields of biology and medicine as such 3D structures grown from tumor cells recapitulate better tumor characteristics, making these tumoroids unique for personalized cancer research. Assessment of their functional behavior, particularly protein secretion, is of significant importance to provide comprehensive insights. Here, a label-free spectroscopic imaging platform is presented with advanced integrated optofluidic nanoplasmonic biosensor that enables real-time secretion analysis from single tumoroids. A novel two-layer microwell design isolates tumoroids, preventing signal interference, and the
microarray configuration allows concurrent analysis of multiple tumoroids. The dual imaging capability combining time-lapse plasmonic spectroscopy and bright-field microscopy facilitates simultaneous observation of secretion dynamics, motility, and morphology. The integrated biosensor is demonstrated with colorectal tumoroids derived from both cell lines and patient samples to investigate their vascular endothelial growth factor A (VEGF-A) secretion, growth, and movement under various conditions, including normoxia, hypoxia, and drug treatment. This platform, by offering a label-free approach with nanophotonics to monitor tumoroids, can pave the way for new applications in fundamental biological studies, drug screening, and the development of therapies.