MicroRNAs(miRNA)参与癌变过程,包括子宫内膜癌(EC)的发展。本研究旨在探讨三种miRNA(miR-21-5p,miR-205-5p,和miR-222-3p)在子宫内膜癌组织中。此外,SNORD48和U6的表达稳定性,最初计划将其用作标准化的参考miRNA,被调查。子宫内膜组织是从子宫切除术中的111例EC患者和19例接受子宫肌瘤或盆腔器官脱垂手术的患者中获得的,作为对照组,没有肿瘤改变。我们的研究基于数字PCR方法进行的计算(Qiagen,希尔登,德国)来衡量绝对表达。在子宫内膜癌组织中,miR-205-5p上调,而miR-222-3p和SNORD48与对照组相比下调。我们检测到miR-205-5p的统计学显著相关性,U6和SNORD48表达具有不同的组织学等级;miR-205-5p的表达随着组织病理学等级的提高而增加(上皮内瘤变-EIN=1590,G1=3367.2,G2=8067和G3=20,360),而U6和SNORD表达从EIN降低到G2,并在G3等级中再次升高(U6:EIN=19,032,G1=16,482.4,G2=13,642.4,G3=133,008;SNORD48:EIN=97,088,G1=59,520,G2=43,544,G3=227,200)。我们的研究表明,miR-205-5p的上调和miR-222-3p和SNORD48的下调可能会影响子宫内膜癌的发展。此外,miR-205-5p,U6和SNORD48的表达变化可能与子宫内膜癌的进展有关。结果还表明,SNORD48和U6通常用作内部参考,可能影响子宫内膜癌的发展和进展;因此,它们不应该被用作参考。然而,值得注意的是,需要进一步研究以了解它们在子宫内膜癌中的作用。
MicroRNAs (miRNA) are involved in the process of carcinogenesis, including the development of endometrial cancer (EC). This study aimed to investigate the association between the expression of three miRNAs (miR-21-5p, miR-205-5p, and miR-222-3p) in endometrial cancer tissues. In addition, the stability of expression of SNORD48 and U6, which were initially planned to be used as reference miRNAs for normalization, was investigated. Endometrial tissue was obtained from 111 patients with EC during hysterectomy and from 19 patients undergoing surgery for uterine fibroids or pelvic organ prolapse as a control group without neoplastic changes. Our study was based on calculations made with a digital PCR method (Qiagen, Hilden, Germany) to measure the absolute expression. In the endometrial cancer tissue, miR-205-5p was upregulated, while miR-222-3p and SNORD48 were downregulated compared to the control group. We detected statistically significant correlation of miR-205-5p, U6, and SNORD48 expression with different histological grades; the expression of miR-205-5p increases with the histopathological grade advancement (intraepithelial neoplasia- EIN = 1590, G1 = 3367.2, G2 = 8067 and G3 = 20,360), while U6 and SNORD expression decreases from EIN to G2 and increases again in the G3 grade (U6: EIN = 19,032, G1 = 16,482.4, G2 = 13,642.4, G3 = 133,008; SNORD48: EIN = 97,088, G1 = 59,520, G2 = 43,544, G3 = 227,200). Our study suggests that upregulation of miR-205-5p and downregulation of miR-222-3p and SNORD48 may influence development of endometrial cancer. Moreover, miR-205-5p, U6, and SNORD48 expression changes may be associated with progression of endometrial cancer. The results also indicate that SNORD48 and U6, commonly used as internal references, may influence endometrial cancer development and progression; therefore, they should not be used as references. However, it is important to note that further research is required to understand their role in endometrial cancer.